AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 273: F1039-F1047, 1997;
0363-6127/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brosius, F. C.
Right arrow Articles by Alper, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brosius, F. C., III
Right arrow Articles by Alper, S. L.

AJP - Renal Physiology, Vol 273, Issue 6 1039-F1047, Copyright © 1997 by American Physiological Society

ARTICLES

AE anion exchanger mRNA and protein expression in vascular smooth muscle cells, aorta, and renal microvessels

F. C. Brosius 3rd, R. L. Pisoni, X. Cao, G. Deshmukh, D. Yannoukakos, A. K. Stuart-Tilley, C. Haller and S. L. Alper
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109-0676, USA.

Intracellular pH (pHi) is an important regulator of vascular smooth muscle cell (VSMC) tone, contractility, and intracellular Ca2+ concentration. Among the multiple transport processes that regulate VSMC pHi, Na(+)-independent Cl-/HCO3- exchange is the major process that acidifies VSMCs in response to an alkaline load. Here, we characterize, in native and cultured VSMCs, the expression of the AE family of band 3-related anion exchangers, the best studied of these Cl-/HCO3- exchangers. A 4.2-kb AE2 mRNA was present in aorta and in all cultured VSMCs tested. Cultured VSMCs and aorta both expressed a approximately 165-kDa AE2 polypeptide, but a approximately 115-kDa polypeptide was the major AE2-related protein in aorta. AE3 mRNA levels in VSMCs and in arterial tissue were significantly lower than those for AE2, but AE3 or related polypeptides were readily detected by immunoblot and immunolocalization experiments. The approximately 125-kDa AE3 polypeptide was present in an immortalized aortic VSMC line, but the predominant AE3 epitope in aorta and most cultured cells was associated with a polypeptide of M(r) approximately 80 kDa. These data demonstrate the expression in native arteries and in VSMCs of products of the AE2 and AE3 genes, which may contribute to Na(+)-independent Cl-/HCO3- exchange activity in these tissues and cells.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Komlosi, S. Frische, A. L. Fuson, A. Fintha, A. Zsembery, J. Peti-Peterdi, and P. D. Bell
Characterization of basolateral chloride/bicarbonate exchange in macula densa cells
Am J Physiol Renal Physiol, February 1, 2005; 288(2): F380 - F386.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Frische, A. S. Zolotarev, Y.-H. Kim, J. Praetorius, S. Alper, S. Nielsen, and S. M. Wall
AE2 isoforms in rat kidney: immunohistochemical localization and regulation in response to chronic NH4Cl loading
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1163 - F1170.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. L. Alper, H. Rossmann, S. Wilhelm, A. K. Stuart-Tilley, B. E. Shmukler, and U. Seidler
Expression of AE2 anion exchanger in mouse intestine
Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G321 - G332.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online