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AJP - Renal Physiology, Vol 273, Issue 6 949-F953, Copyright © 1997 by American Physiological Society
ARTICLES |
J. D. Klein, S. R. Price, J. L. Bailey, J. D. Jacobs and J. M. Sands
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Providing glucocorticoids to adrenalectomized (Adx) rats results in downregulation of the vasopressin (AVP)-regulated urea transporter (VRUT) in the renal inner medullary (IM) tip. To examine the physiological relevance of this response, we studied rats with uncontrolled diabetes mellitus induced by streptozotocin (STZ), since these rats have increased corticosterone production and urea excretion. We measured VRUT protein in extracts from the IM tip or base of pair-fed control and diabetic rats by Western analysis using an antibody to rat VRUT. In the IM tip, VRUT was significantly reduced by 39% in diabetic compared with control rats. In the IM base, there was no significant difference between diabetic and control rats. To determine whether the decrease in VRUT in the IM tip was mediated by glucocorticoids, the experiment was repeated using the following three groups of rats: 1) Adx alone, 2) Adx + STZ, and 3) Adx + STZ + replacement with a physiological dose of glucocorticoid. There was no significant difference in VRUT between Adx and Adx + STZ rats. However, VRUT was significantly reduced by 32% in the IM tip of glucocorticoid-treated Adx + STZ rats compared with control Adx + STZ rats. We conclude that glucocorticoids regulate the abundance of VRUT protein independently of insulin in diabetic rats.
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  A. C. Mistry, R. Mallick, J. D. Klein, T. Weimbs, J. M. Sands, and O. Frohlich Syntaxin specificity of aquaporins in the inner medullary collecting duct Am J Physiol Renal Physiol, August 1, 2009; 297(2): F292 - F300. [Abstract] [Full Text] [PDF]
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N. W. Blessing, M. A. Blount, J. M. Sands, C. F. Martin, and J. D. Klein Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1336 - F1341. [Abstract] [Full Text] [PDF]
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M. A. Blount, J. M. Sands, K. J. Kent, T. D. Smith, S. R. Price, and J. D. Klein Candesartan augments compensatory changes in medullary transport proteins in the diabetic rat kidney Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1448 - F1452. [Abstract] [Full Text] [PDF]
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M. A. Blount, J. D. Klein, C. F. Martin, D. Tchapyjnikov, and J. M. Sands Forskolin stimulates phosphorylation and membrane accumulation of UT-A3 Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1308 - F1313. [Abstract] [Full Text] [PDF]
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J. D. Klein, B. P. Murrell, S. Tucker, Y.-H. Kim, and J. M. Sands Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension Am J Physiol Renal Physiol, November 1, 2006; 291(5): F952 - F959. [Abstract] [Full Text] [PDF]
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 J. D. Klein, O. Frohlich, M. A. Blount, C. F. Martin, T. D. Smith, and J. M. Sands Vasopressin Increases Plasma Membrane Accumulation of Urea Transporter UT-A1 in Rat Inner Medullary Collecting Ducts J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2680 - 2686. [Abstract] [Full Text] [PDF]
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 J. J. Doran, J. D. Klein, Y. H. Kim, T. D. Smith, S. D. Kozlowski, R. B. Gunn, and J. M. Sands Tissue distribution of UT-A and UT-B mRNA and protein in rat Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1446 - R1459. [Abstract] [Full Text] [PDF]
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S.-W. Lim, K.-H. Han, J.-Y. Jung, W.-Y. Kim, C.-W. Yang, J. M. Sands, M. A. Knepper, K. M. Madsen, and J. Kim Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R479 - R492. [Abstract] [Full Text] [PDF]
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D. Kim, J. D. Klein, S. Racine, B. P. Murrell, and J. M. Sands Urea may regulate urea transporter protein abundance during osmotic diuresis Am J Physiol Renal Physiol, January 1, 2005; 288(1): F188 - F197. [Abstract] [Full Text] [PDF]
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D. Kim, J. M. Sands, and J. D. Klein Role of vasopressin in diabetes mellitus-induced changes in medullary transport proteins involved in urine concentration in Brattleboro rats Am J Physiol Renal Physiol, April 1, 2004; 286(4): F760 - F766. [Abstract] [Full Text] [PDF]
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R. A. Gertner, J. D. Klein, J. L. Bailey, D.-u. Kim, X. H. Luo, S. M. Bagnasco, and J. M. Sands Aldosterone Decreases UT-A1 Urea Transporter Expression via the Mineralocorticoid Receptor J. Am. Soc. Nephrol., March 1, 2004; 15(3): 558 - 565. [Abstract] [Full Text] [PDF]
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D. Kim, J. M. Sands, and J. D. Klein Changes in renal medullary transport proteins during uncontrolled diabetes mellitus in rats Am J Physiol Renal Physiol, August 1, 2003; 285(2): F303 - F309. [Abstract] [Full Text] [PDF]
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S. Combet, N. Geffroy, V. Berthonaud, B. Dick, L. Teillet, J.-M. Verbavatz, B. Corman, and M.-M. Trinh-Trang-Tan Correction of age-related polyuria by dDAVP: molecular analysis of aquaporins and urea transporters Am J Physiol Renal Physiol, January 1, 2003; 284(1): F199 - F208. [Abstract] [Full Text] [PDF]
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 R. Duchesne, J. D. Klein, J. B. Velotta, J. J. Doran, P. Rouillard, B. R. Roberts, A. A. McDonough, and J. M. Sands UT-A Urea Transporter Protein in Heart: Increased Abundance During Uremia, Hypertension, and Heart Failure Circ. Res., July 20, 2001; 89(2): 139 - 145. [Abstract] [Full Text] [PDF]
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