| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Urology,
Although most cell membranes permit rapid flux of water, small nonelectrolytes, and ammonia, the apical membranes of bladder epithelial umbrella cells, which form the bladder permeability barrier, exhibit strikingly low permeabilities to these substances. In cystitis, disruption of the bladder permeability barrier may irritate the bladder wall layers underlying the epithelium, causing or exacerbating inflammation, and increasing urinary frequency, urgency, and bladder pain. To determine the effects of inflammation on the integrity of the permeability barrier, guinea pigs were sensitized with ovalbumin, and the bladders were exposed subsequently to antigen by instillation on the urinary side. Inflammation of the bladder wall markedly reduced transepithelial resistance of dissected epithelium mounted in Ussing chambers and increased water and urea permeabilities modestly at 2 h and more strikingly at 24 h after induction of the inflammation. Transmission and scanning electron microscopy of bladders at 30 min and 24 h after antigen exposure revealed disruption of tight junctions, denuding of patches of epithelium, and occasional loss of apical membrane architecture. These permeability and structural effects did not occur in nonsensitized animals in which the bladders were exposed to antigen and in sensitized animals exposed to saline vehicle rather than antigen. These results demonstrate that inflammation of the underlying muscle and lamina propria can disrupt the bladder permeability barrier by damaging tight junctions and apical membranes and causing sloughing of epithelial cells. Leakage of urinary constituents through the damaged epithelium may then exacerbate the inflammation in the underlying muscle layers.
water transport; membrane permeability; epithelial cell damage
This article has been cited by other articles:
|
|
 |
|
  W. G. Hill, S. Meyers, M. von Bodungen, G. Apodaca, J. R. Dedman, M. A. Kaetzel, and M. L. Zeidel Studies on localization and function of annexin A4a within urinary bladder epithelium using a mouse knockout model Am J Physiol Renal Physiol, April 1, 2008; 294(4): F919 - F927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Endeward and G. Gros Low carbon dioxide permeability of the apical epithelial membrane of guinea-pig colon J. Physiol., August 15, 2005; 567(1): 253 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Cross, I. Eardley, H. J. Leese, and J. Southgate A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function Am J Physiol Renal Physiol, August 1, 2005; 289(2): F459 - F468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Apodaca, S. Kiss, W. Ruiz, S. Meyers, M. Zeidel, and L. Birder Disruption of bladder epithelium barrier function after spinal cord injury Am J Physiol Renal Physiol, May 1, 2003; 284(5): F966 - F976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Kanai, M. L. Zeidel, J. P. Lavelle, J. S. Greenberger, L. A. Birder, W. C. De Groat, G. L. Apodaca, S. A. Meyers, R. Ramage, and M. W. Epperly Manganese superoxide dismutase gene therapy protects against irradiation-induced cystitis Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1304 - F1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hu, S. Meyers, F.-X. Liang, F.-M. Deng, B. Kachar, M. L. Zeidel, and T.-T. Sun Role of membrane proteins in permeability barrier function: uroplakin ablation elevates urothelial permeability Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1200 - F1207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lavelle, S. Meyers, R. Ramage, S. Bastacky, D. Doty, G. Apodaca, and M. L. Zeidel Bladder permeability barrier: recovery from selective injury of surface epithelial cells Am J Physiol Renal Physiol, August 1, 2002; 283(2): F242 - F253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Saban, N.-B. Nguyen, T. G. Hammond, and R. Saban Gene Expression Profiling of Mouse Bladder Inflammatory Responses to LPS, Substance P, and Antigen-Stimulation Am. J. Pathol., June 1, 2002; 160(6): 2095 - 2110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Shochina, Y Fellig, M Sughayer, G Pizov, K Vitner, D Podeh, A Hochberg, and I Ariel Nitric oxide synthase immunoreactivity in human bladder carcinoma Mol. Pathol., August 1, 2001; 54(4): 248 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Lewis Everything you wanted to know about the bladder epithelium but were afraid to ask Am J Physiol Renal Physiol, June 1, 2000; 278(6): F867 - F874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Lavelle, S. A. Meyers, W. G. Ruiz, C. A. T. Buffington, M. L. Zeidel, and G. Apodaca Urothelial pathophysiological changes in feline interstitial cystitis: a human model Am J Physiol Renal Physiol, April 1, 2000; 278(4): F540 - F553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Saban, M. R. Saban, N.-B. Nguyen, B. Lu, C. Gerard, N. P. Gerard, and T. G. Hammond Neurokinin-1 (NK-1) Receptor Is Required in Antigen-Induced Cystitis Am. J. Pathol., March 1, 2000; 156(3): 775 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Hill and M. L. Zeidel Reconstituting the Barrier Properties of a Water-tight Epithelial Membrane by Design of Leaflet-specific Liposomes J. Biol. Chem., September 22, 2000; 275(39): 30176 - 30185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. SABAN, M. R. SABAN, N.-B. NGUYEN, T. G. HAMMOND, and B. K. WERSHIL Mast cell regulation of inflammation and gene expression during antigen-induced bladder inflammation in mice Physiol Genomics, October 10, 2001; 7(1): 35 - 43. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
