AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 274: F43-F50, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oliverio, M. I.
Right arrow Articles by Coffman, T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oliverio, M. I.
Right arrow Articles by Coffman, T. M.
Vol. 274, Issue 1, F43-F50, January 1998

Renal growth and development in mice lacking AT1A receptors for angiotensin II

Michael I. Oliverio1, Kirsten Madsen3, Christopher F. Best1, Masaki Ito2, Nobuyo Maeda4, Oliver Smithies4, and Thomas M. Coffman1

1 Department of Medicine, Duke University and Durham Veterans Affair Medical Centers, Durham, North Carolina 27705; 2 Department of Clinical Pharmacology and Therapeutics, University of Shizuoka, Shizuoka, Japan; 3 Division of Nephrology, Hypertension, and Transplantation, University of Florida, Gainesville, Florida 32610; and 4 Department of Pathology, University of North Carolina, Chapel Hill, North Carolina 27599

To examine the role of the type 1A (AT1A) angiotensin receptor in renal growth and development, we analyzed F2 progeny from a series of crosses between F1 mice that were heterozygous for a targeted disruption of the AT1A receptor gene [Agtr1A-(+/-)]. Among 21-day-old weanling F2 mice, we found that 194 (32%) were homozygous for the wild-type allele Agtr1A-(+/+), 299 (49%) were Agtr1A-(+/-), and 119 (19%) were Agtr1A-(-/-). This differed significantly from the proportions predicted by Mendelian genetics (P = 0.01), suggesting that the complete absence of AT1A receptors is associated with a mild survival disadvantage. Agtr1A-(-/-) mice grew normally, and we found no significant differences in body weight or heart and kidney weights in Agtr1A-(+/+) and Agtr1A-(-/-) mice examined at 21, 60, and 100 days. Protein and DNA content of kidneys and hearts were also similar in weanling or adult Agtr1A-(+/+) and Agtr1A-(-/-) mice. By light microscopy with immunohistochemistry, kidneys from Agtr1A-(-/-) were essentially normal, with two exceptions: 1) there was marked hypertrophy of the juxtaglomerular apparatus (JGA) and proximal expansion of renin-producing cells along the afferent arterioles, and 2) some glomeruli showed evidence of mesangial expansion. We did not find the severe renal vascular lesions or papillary atrophy that have been observed in angiotensinogen- or angiotensin converting enzyme-deficient animals. We conclude that the AT1A receptor is not essential for the normal organogenesis of the kidney; however, its absence is associated with mild mesangial expansion and JGA hypertrophy.

renin; gene targeting; kidney; mesangium; juxtaglomerular apparatus


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
X. C. Li and J. L. Zhuo
In vivo regulation of AT1a receptor-mediated intracellular uptake of [125I]Val5-ANG II in the kidneys and adrenals of AT1a receptor-deficient mice
Am J Physiol Renal Physiol, February 1, 2008; 294(2): F293 - F302.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. C. Li, L. G. Navar, Y. Shao, and J. L. Zhuo
Genetic deletion of AT1a receptors attenuates intracellular accumulation of ANG II in the kidney of AT1a receptor-deficient mice
Am J Physiol Renal Physiol, August 1, 2007; 293(2): F586 - F593.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. O. Topcu, M. Pedersen, R. Norregaard, G. Wang, M. Knepper, J. C. Djurhuus, S. Nielsen, T. M. Jorgensen, and J. Frokiaer
Candesartan prevents long-term impairment of renal function in response to neonatal partial unilateral ureteral obstruction
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F736 - F748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. M. Harrison-Bernard, C. J. Monjure, and B. J. Bivona
Efferent arterioles exclusively express the subtype 1A angiotensin receptor: functional insights from genetic mouse models
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1177 - F1186.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
L. J. Mullins, M. A. Bailey, and J. J. Mullins
Hypertension, Kidney, and Transgenics: A Fresh Perspective
Physiol Rev, April 1, 2006; 86(2): 709 - 746.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
S. H. Padia, N. L. Howell, H. M. Siragy, and R. M. Carey
Renal Angiotensin Type 2 Receptors Mediate Natriuresis Via Angiotensin III in the Angiotensin II Type 1 Receptor-Blocked Rat
Hypertension, March 1, 2006; 47(3): 537 - 544.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, C. W. Yang, J. H. Park, S. W. Lim, B. K. Sun, J. Y. Jung, S. B. Kim, Y. S. Kim, J. Kim, and B. K. Bang
Pravastatin treatment attenuates interstitial inflammation and fibrosis in a rat model of chronic cyclosporine-induced nephropathy
Am J Physiol Renal Physiol, January 1, 2004; 286(1): F46 - F57.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
M. Maeda, A. Yabuki, S. Suzuki, M. Matsumoto, K. Taniguchi, and H. Nishinakagawa
Renal Lesions in Spontaneous Insulin-dependent Diabetes Mellitus in the Nonobese Diabetic Mouse: Acute Phase of Diabetes
Vet. Pathol., March 1, 2003; 40(2): 187 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. M. Harrison-Bernard, A. K. Cook, M. I. Oliverio, and T. M. Coffman
Renal segmental microvascular responses to ANG II in AT1A receptor null mice
Am J Physiol Renal Physiol, March 1, 2003; 284(3): F538 - F545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, C. W. Yang, W. Y. Kim, J. Y. Jung, J. H. Cha, Y. S. Kim, J. Kim, W. M. Bennett, and B. K. Bang
Reversibility of chronic cyclosporine nephropathy in rats after withdrawal of cyclosporine
Am J Physiol Renal Physiol, February 1, 2003; 284(2): F389 - F398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. J. Mangrum, R. A. Gomez, and V. F. Norwood
Effects of AT1A receptor deletion on blood pressure and sodium excretion during altered dietary salt intake
Am J Physiol Renal Physiol, September 1, 2002; 283(3): F447 - F453.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. C. Cholewa and D. L. Mattson
Role of the renin-angiotensin system during alterations of sodium intake in conscious mice
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2001; 281(3): R987 - R993.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger
International Union of Pharmacology. XXIII. The Angiotensin II Receptors
Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
X. RUAN, M. I. OLIVERIO, T. M. COFFMAN, and W. J. ARENDSHORST
Renal Vascular Reactivity in Mice: AngII-Induced Vasoconstriction inAT1A Receptor Null Mice
J. Am. Soc. Nephrol., December 1, 1999; 10(12): 2620 - 2630.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. E. Stec, R. L. Davisson, R. E. Haskell, B. L. Davidson, and C. D. Sigmund
Efficient Liver-specific Deletion of a Floxed Human Angiotensinogen Transgene by Adenoviral Delivery of Cre Recombinase in Vivo
J. Biol. Chem., July 23, 1999; 274(30): 21285 - 21290.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. M. Coffman
Gene targeting in physiological investigations: studies of the renin-angiotensin system
Am J Physiol Renal Physiol, June 1, 1998; 274(6): F999 - F1005.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online