| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
/
exchanger in the basolateral membrane of mouse medullary thick
ascending limb
Division of Renal Diseases, Rhode Island Hospital and Department of Medicine, Brown University School of Medicine, Providence, Rhode Island 02903
Although a basolateral
Cl
/
exchanger (AE) has been implicated in the arginine vasopressin
(AVP)-dependent hypertonic regulatory increase in the medullary thick
ascending limb (MTAL), there are conflicting data regarding whether
this exchanger is indeed present in this tubule segment. In this study, mouse MTAL was examined whether
Cl/
exchange activity was present in the basolateral membrane and whether
mRNAs from the known AE genes are expressed. Cl/
exchange activity was examined in isolated perfused MTAL tubules under
isotonic conditions and in the absence of arginine vasopressin.
2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein was used
to monitor intracellular pH. Removal of basolateral
Cl induced reversible cell
alkalization that was independent of external
Na+ and completely inhibited by
peritubular 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (200 µM). The rate and extent of cell alkalinization were significantly greater in the presence than absence of external CO2/.
A voltage clamp did not inhibit cell alkalinization induced by
basolateral Cl removal.
Consistently, addition of basolateral
Cl induced reversible cell
acidification in MTAL depleted of intracellular Cl. Furthermore, mRNA
encoding two members (AE2 and AE3) of the AE gene family were
demonstrated in microdissected mouse MTAL tubules by reverse
transcription-polymerase chain reaction. It is concluded that AE is
present in the basolateral membrane of mouse MTAL.
chloride ion; bicarbonate; regulatory volume increase; reverse transcription-polymerase chain reaction
This article has been cited by other articles:
|
|
 |
|
  P. L. Dudas, S. Mentone, C. F. Greineder, D. Biemesderfer, and P. S. Aronson Immunolocalization of anion transporter Slc26a7 in mouse kidney Am J Physiol Renal Physiol, April 1, 2006; 290(4): F937 - F945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Watts III and D. W. Good An apical K+-dependent HCO3- transport pathway opposes transepithelial HCO3- absorption in rat medullary thick ascending limb Am J Physiol Renal Physiol, July 1, 2004; 287(1): F57 - F63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bourgeois, S. Masse, M. Paillard, and P. Houillier Basolateral membrane Cl--, Na+-, and K+-coupled base transport mechanisms in rat MTALH Am J Physiol Renal Physiol, April 1, 2002; 282(4): F655 - F668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shah, R. Quigley, and M. Baum Neonatal rabbit proximal tubule basolateral membrane Na+/H+ antiporter and Cl-/base exchange Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1999; 276(6): R1792 - R1797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Leviel, D. Eladari, A. Blanchard, J.-S. Poumarat, M. Paillard, and R.-A. Podevin Pathways for HCO-3 exit across the basolateral membrane in rat thick limbs Am J Physiol Renal Physiol, June 1, 1999; 276(6): F847 - F856. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
