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Institut für Anatomie und Zellbiologie I, Universität Heidelberg, Im Neuenheimer Feld 307, D-69120 Heidelberg; Max-Planck-Institut für molekulare Physiologie, Rheinlanddamm 201, D-44139 Dortmund, Germany; and Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672
Localization of a recently described and cloned Na-Pi cotransport system from flounder was investigated by reverse transcription-polymerase chain reaction (RT-PCR) of microdissected tubules and by immunocytochemistry of kidney of winter flounder. Histological examination showed a small glomerulus, an extremely short proximal tubule PI with a selective affinity to Lens culinaris agglutinin from lentils, and an extensive second proximal tubule segment PII (>90% of proximal tubules), consisting of cells with numerous apical clear vesicles and extensive amplification of basolateral cell membranes. PII merged with the collecting tubule/collecting duct (CT/CD) system without a distal segment. By RT-PCR, PII cells revealed high levels of NaPi-II related RNA; low levels were also observed in CTs. Previously characterized antisera against different epitopes of flounder NaPi-II specifically labeled the basolateral regions of PII and the apical cell portion of CT/CD cells and of some PII cells. These results suggest that tubular secretion of Pi occurs in PII of teleost fish with modulation of urinary Pi content in the subsequent CT/CD system.
proximal tubule; immunocytochemistry; reverse transcription-polymerase chain reaction; sodium-phosphate cotransporter; collecting duct; flounder kidney
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