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Am J Physiol Renal Physiol 274: F453-F462, 1998;
0363-6127/98 $5.00
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Vol. 274, Issue 3, F453-F462, March 1998

Water and solute permeabilities of medullary thick ascending limb apical and basolateral membranes

Rickey Rivers, Anne Blanchard, Dominique Eladari, Francois Leviel, Michel Paillard, Rene-Alexandre Podevin, and Mark L. Zeidel

Laboratory of Epithelial Cell Biology, Renal-Electrolyte Division, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213; and Laboratoire de Physiologie et Endocrinologie Cellulaire et Moleculaire, Université Pierre et Marie Curie, Faculté de Medecine, Broussais-Hotel Dieu and Institut National de la Santé et de la Recherche Médicale U356, Paris, France

The medullary thick ascending limb (MTAL) reabsorbs solute without water and concentrates NH+4 in the interstitium without a favorable pH gradient, activities which require low water and NH3 permeabilities. The contributions of different apical and basolateral membrane structures to these low permeabilities are unclear. We isolated highly purified apical and basolateral MTAL plasma membranes and measured, by stopped-flow fluorometry, their permeabilities to water, urea, glycerol, protons, and NH3. Osmotic water permeability at 20°C averaged 9.4 ± 0.8 × 10-4 cm/s for apical and 11.9 ± 0.5 × 10-4 cm/s for basolateral membranes. NH3 permeabilities at 20°C averaged 0.0023 ± 0.00035 and 0.0035 ± 0.00080 cm/s for apical and basolateral membranes, respectively. These values are consistent with those obtained in isolated perfused tubules and can account for known aspects of MTAL function in vivo. Because the apical and basolateral membrane unit permeabilities are similar, the ability of the apical membrane to function as the site of barrier function arises from its very small surface area when compared with the highly redundant basolateral membrane.

kidney; medulla; medullary thick ascending limb; water permeability; renal concentrating mechanism; renal ammonium transport


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