Vol. 274, Issue 3, F490-F497, March 1998
The endogenous insulin-like growth factor system in
radiocontrast nephropathy
Z.
Symon,
S.
Fuchs,
Y.
Agmon,
O.
Weiss,
I.
Nephesh,
R.
Moshe,
M.
Brezis,
A.
Flyvbjerg, and
I.
Raz
Department of Medicine, Hadassah University Hospital, Jerusalem,
Israel; and Institute of Experimental Clinical Research, Aarhus
Kommunehospital, Aarhus C, Denmark
The response of insulin-like growth factor (IGF) I in acute
renal failure was evaluated in a model of radiocontrast nephropathy associated with selective necrosis of medullary thick ascending limbs.
In brief, rats were administered radiocontrast medium or vehicle
injections for controls after combined inhibition of prostanoids and
nitric oxide. Twenty-four hours after the insult, tissue mRNAs for
IGF-I, the IGF-I receptor, and IGF-binding proteins (IGFBP) 1 and 3 were assayed in cortex, medulla, and liver by solution hybridization-RNase protection assay, and IGFBPs were measured in serum
and tissue by Western ligand blotting. Cortical IGF-I increased,
whereas medullary IGF-I mRNA decreased. Renal IGFBPs decreased, whereas
IGFBP-1 mRNA increased. The IGF system in the liver was unchanged. We
conclude that general changes in renal IGFBPs in this experimental
model of acute renal failure might increase the level of cortical IGF-I
in a way that could modulate medullary recovery.
acute renal failure; nitric oxide; prostanoids
