| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7545
Calcium signaling
mechanisms were examined in vessel segments and dispersed single smooth
muscle cells (SMC) of interlobular arteries and afferent arterioles
(<50 µm diameter) from the rat kidney. These resistance vessels
were isolated from rat kidneys, using an iron oxide-sieving technique
with subsequent collagenase digestion. Individual cells were identified
by their characteristic oval appearance and positive staining for
smooth muscle-specific 
-actin and heavy chain myosin SM-1 and SM-2.
Cytosolic calcium concentration
([Ca2+]i)
was measured using fura 2 ratiometric fluorescence at 340 and 380 nm
wavelength with a microscope-based photometer. Angiotensin II (ANG II)
and arginine vasopressin (AVP), at concentrations of
10
10-106
M, produced dose-dependent increases in
[Ca2+]i;
maximum increases were 221 ± 49 nM for ANG II and 237 ± 49 nM
for AVP. The temporal response patterns for both agonists were characterized by a square-shaped, immediate step increase in
[Ca2+]i
to a near maximum level that was maintained through the recording period of 150-200 s. Responses of individual dispersed SMC and short vessel segments were similar. Losartan antagonized the action of
ANG II, indicating mediation by
AT1 receptors on preglomerular arteriolar SMC. The V1-selective
antagonist
[d(CH2)5Tyr(Me)2Tyr(NH2)9]AVP completely inhibited AVP-induced
[Ca2+]i
changes. The importance of calcium entry in hormone-induced changes in
[Ca2+]i
was demonstrated by the finding that neither ANG II nor AVP elicited a
[Ca2+]i
response in media rendered nominally calcium free by addition of
ethylene glycol-bis(
-aminoethyl
ether)-N,N,N',N'-tetraacetic acid. Calcium entry occurred primarily through L-type, voltage-gated calcium channels as the dihydropyridine, nifedipine, completely prevented or reversed
[Ca2+]i
changes normally elicited by either hormone. Our results provide new
information about the similarity of calcium signaling in single SMC and
short segments freshly isolated from renal interlobular arteries and
afferent arterioles. The observations indicate that AT1 and
V1 receptors are coupled to signal
transduction pathways leading to rapid changes in
[Ca2+]i.
Calcium mobilization appears to play a minor to nonexistent role under
the experimental conditions. The predominant mechanism involves calcium
entry through dihydropyridine-sensitive, voltage-gated calcium channels
in single SMC from these resistance vessels.
kidney; renal circulation; glomerulus; vascular smooth muscle; interlobular artery; afferent arteriole; cytosolic calcium concentration; fura 2; calcium channel blockers; dihydropyridine; L-type calcium channels; receptor-operated calcium channel; AT1 receptor; V1 receptor; losartan
This article has been cited by other articles:
|
|
 |
|
  Q. Zhang, C. Cao, Z. Zhang, W. G. Wier, A. Edwards, and T. L. Pallone Membrane current oscillations in descending vasa recta pericytes Am J Physiol Renal Physiol, March 1, 2008; 294(3): F656 - F666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Magnusson, C. M. Sorensen, T. H. Braunstein, N.-H. Holstein-Rathlou, and M. Salomonsson Renovascular BKCa channels are not activated in vivo under resting conditions and during agonist stimulation Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R345 - R353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Helle, O. B. Vagnes, and B. M. Iversen Angiotensin II-induced calcium signaling in the afferent arteriole from rats with two-kidney, one-clip hypertension Am J Physiol Renal Physiol, July 1, 2006; 291(1): F140 - F147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilcox Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Fuller, B. C. Hauschild, R. Gonzalez-Villalobos, M. S. Awayda, J. D. Imig, E. W. Inscho, and L. G. Navar Calcium and chloride channel activation by angiotensin II-AT1 receptors in preglomerular vascular smooth muscle cells Am J Physiol Renal Physiol, October 1, 2005; 289(4): F760 - F767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. H. Hansen, O. B. Vagnes, and B. M. Iversen Enhanced response to AVP in the interlobular artery from the spontaneously hypertensive rat Am J Physiol Renal Physiol, May 1, 2005; 288(5): F1023 - F1031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Fallet, H. Ikenaga, J. P. Bast, and P. K. Carmines Relative contributions of Ca2+ mobilization and influx in renal arteriolar contractile responses to arginine vasopressin Am J Physiol Renal Physiol, March 1, 2005; 288(3): F545 - F551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Sorensen, P. P. Leyssac, M. Salomonsson, O. Skott, and N.-H. Holstein-Rathlou ANG II-induced downregulation of RBF after a prolonged reduction of renal perfusion pressure is due to pre- and postglomerular constriction Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2004; 286(5): R865 - R873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Salomonsson and W. J. Arendshorst Effect of tyrosine kinase blockade on norepinephrine-induced cytosolic calcium response in rat afferent arterioles Am J Physiol Renal Physiol, May 1, 2004; 286(5): F866 - F874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Facemire, P. J. Mohler, and W. J. Arendshorst Expression and relative abundance of short transient receptor potential channels in the rat renal microcirculation Am J Physiol Renal Physiol, March 1, 2004; 286(3): F546 - F551. [Abstract] [Full Text]
|
|
|
|
|
|
J. Steendahl, N.-H. Holstein-Rathlou, C. M. Sorensen, and M. Salomonsson Effects of chloride channel blockers on rat renal vascular responses to angiotensin II and norepinephrine Am J Physiol Renal Physiol, February 1, 2004; 286(2): F323 - F330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Che and P. K. Carmines Angiotensin II Triggers EGFR Tyrosine Kinase-Dependent Ca2+ Influx in Afferent Arterioles Hypertension, November 1, 2002; 40(5): 700 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Salomonsson, F. Gustafsson, D. Andreasen, B. L. Jensen, and N.-H. Holstein-Rathlou Local electric stimulation causes conducted calcium response in rat interlobular arteries Am J Physiol Renal Physiol, September 1, 2002; 283(3): F473 - F480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Salomonsson and W. J. Arendshorst Norepinephrine-induced calcium signaling pathways in afferent arterioles of genetically hypertensive rats Am J Physiol Renal Physiol, August 1, 2001; 281(2): F264 - F272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Touyz and E. L. Schiffrin Signal Transduction Mechanisms Mediating the Physiological and Pathophysiological Actions of Angiotensin II in Vascular Smooth Muscle Cells Pharmacol. Rev., December 1, 2000; 52(4): 639 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Loutzenhiser and R. Loutzenhiser Angiotensin II-Induced Ca2+ Influx in Renal Afferent and Efferent Arterioles : Differing Roles of Voltage-Gated and Store-Operated Ca2+ Entry Circ. Res., September 29, 2000; 87(7): 551 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kostrzewska, B. Modzelewska, and S. Batra Source of calcium for contractile responses of large and small human intramyometrial arteries Hum. Reprod., September 1, 2000; 15(9): 1927 - 1931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Vagnes, J. J. Feng, B. M. Iversen, and W. J. Arendshorst Upregulation of V1 receptors in renal resistance vessels of rats developing genetic hypertension Am J Physiol Renal Physiol, June 1, 2000; 278(6): F940 - F948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Purdy and W. J. Arendshorst Prostaglandins buffer ANG II-mediated increases in cytosolic calcium in preglomerular VSMC Am J Physiol Renal Physiol, December 1, 1999; 277(6): F850 - F858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Fellner and W. J. Arendshorst Capacitative calcium entry in smooth muscle cells from preglomerular vessels Am J Physiol Renal Physiol, October 1, 1999; 277(4): F533 - F542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Salomonsson and W. J. Arendshorst Calcium recruitment in renal vasculature: NE effects on blood flow and cytosolic calcium concentration Am J Physiol Renal Physiol, May 1, 1999; 276(5): F700 - F710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Iversen and W. J. Arendshorst Exaggerated Ca2+ signaling in preglomerular arteriolar smooth muscle cells of genetically hypertensive rats Am J Physiol Renal Physiol, February 1, 1999; 276(2): F260 - F270. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
