AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 274: F509-F515, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aleo, M. D.
Right arrow Articles by Schnellmann, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aleo, M. D.
Right arrow Articles by Schnellmann, R. G.
Vol. 274, Issue 3, F509-F515, March 1998

Recovery of cellular functions following oxidant injury

Grazyna Nowak1, Michael D. Aleo2, Jan A. Morgan1, and Rick G. Schnellmann1

1 Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205-7199; and 2 Pfizer, Central Research Division, Groton, Connecticut 06340

This study investigated the recovery of renal proximal tubule cellular (RPTC) functions following oxidant-induced sublethal injury. tert-Butylhydroperoxide (TBHP) treatment resulted in 24% cell death and loss 4 h following the exposure. The remaining sublethally injured RPTC proliferated, and monolayer DNA content returned to control values on day 4 following TBHP exposure. Basal oxygen consumption (QO2) and ATP content in sublethally injured RPTC were decreased 64 and 63%, respectively, at 4 h and returned to control values on day 6. Net lactate consumption decreased 71% at 4 h and returned to control values on day 4. In contrast, net glutamine consumption increased 2.7-fold at 4 h and returned to control values on day 6. Ouabain-sensitive QO2, Na+-K+-adenosinetriphosphatase (Na+-K+-ATPase) activity, and Na+-coupled glucose transport were inhibited 77, 88, and 83%, respectively, at 4 h and recovered to control values on day 6. These data show that 1) mitochondrial function, Na+-K+-ATPase activity, active Na+ transport, and Na+-coupled glucose transport are decreased in sublethally injured RPTC following oxidant exposure and are repaired over time; 2) monolayer regeneration precedes the recovery of mitochondrial and transport functions, and 3) sublethal injury and subsequent regeneration are associated with alterations in metabolic substrate utilization. These results suggest that oxidant-induced sublethal injury to RPTC may contribute to renal dysfunction and that RPTC can repair and regain cellular functions following oxidant injury.

renal proximal tubular cells; tert-butylhydroperoxide; cell death; sublethal cell injury; regeneration; cell repair; mitochondrial functions; oxygen consumption; sodium-potassium-adenosinetriphosphatase; active sodium transport; sodium-coupled glucose transport; ascorbic acid; amino acids; glutamine


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
K. A. Rasbach and R. G. Schnellmann
Isoflavones Promote Mitochondrial Biogenesis
J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 536 - 543.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. A. Hallman, S. Zhuang, and R. G. Schnellmann
Regulation of Dedifferentiation and Redifferentiation in Renal Proximal Tubular Cells by the Epidermal Growth Factor Receptor
J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 520 - 528.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
G. Nowak, G. L. Clifton, and D. Bakajsova
Succinate Ameliorates Energy Deficits and Prevents Dysfunction of Complex I in Injured Renal Proximal Tubular Cells
J. Pharmacol. Exp. Ther., March 1, 2008; 324(3): 1155 - 1162.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. H. Lash
Epidermal growth factor receptor ligands and renal epithelial cell proliferation
Am J Physiol Renal Physiol, March 1, 2008; 294(3): F457 - F458.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Z. P. Shaik, E. K. Fifer, and G. Nowak
Akt activation improves oxidative phosphorylation in renal proximal tubular cells following nephrotoxicant injury
Am J Physiol Renal Physiol, February 1, 2008; 294(2): F423 - F432.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Nowak, G. L. Clifton, M. L. Godwin, and D. Bakajsova
Activation of ERK1/2 pathway mediates oxidant-induced decreases in mitochondrial function in renal cells
Am J Physiol Renal Physiol, October 1, 2006; 291(4): F840 - F855.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Zhuang, Y. Dang, and R. G. Schnellmann
Requirement of the epidermal growth factor receptor in renal epithelial cell proliferation and migration
Am J Physiol Renal Physiol, September 1, 2004; 287(3): F365 - F372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Liu, M. L. Godwin, and G. Nowak
Protein kinase C-{alpha} inhibits the repair of oxidative phosphorylation after S-(1,2-dichlorovinyl)-L-cysteine injury in renal cells
Am J Physiol Renal Physiol, July 1, 2004; 287(1): F64 - F73.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Nowak, D. Bakajsova, and G. L. Clifton
Protein kinase C-{epsilon} modulates mitochondrial function and active Na+ transport after oxidant injury in renal cells
Am J Physiol Renal Physiol, February 1, 2004; 286(2): F307 - F316.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
P. A. Nony and R. G. Schnellmann
Mechanisms of Renal Cell Repair and Regeneration after Acute Renal Failure
J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 905 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. A. Nony, G. Nowak, and R. G. Schnellmann
Collagen IV promotes repair of renal cell physiological functions after toxicant injury
Am J Physiol Renal Physiol, September 1, 2001; 281(3): F443 - F453.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. B. Keasler, D. E. McKeller, and R. G. Schnellmann
Differential effects of EGF on repair of cellular functions after dichlorovinyl-L-cysteine-induced injury
Am J Physiol Renal Physiol, February 1, 1999; 276(2): F228 - F236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online