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Division of Nephrology, University of Arkansas for Medical Sciences, and John L. McClellan Memorial Veterans Affairs Hospital, Little Rock, Arkansas 72205
In the present study, we demonstrate that rat kidney contains
caspase activity that was markedly inhibited by specific peptide inhibitors of caspases but not by inhibitors of Ser, Cys, Asp, or
metalloproteinases. Using primers based on the nucleotide sequence of
known members of Ced-3/interleukin-1
-converting enzyme (ICE) family
from human origin, we have identified by reverse-transcription (RT)
polymerase chain reaction (PCR) analyses that rat kidney transcribes
the genes for caspase-1 (ICE), caspase-2 (Nedd2), caspase-3 (CPP32),
and caspase-6 (Mch2). RT-PCR products, when subcloned and
sequenced, provided full-length cDNAs for ICE (1,209 bp) and CPP32 (786 bp) and partial cDNA products for Mch2 (561 bp) and Nedd2 (811 bp). The
sequence analysis of the caspase cDNAs showed conserved catalytic site
QACRG as well as Asp cleavage site. Rat kidneys subjected to
ischemia-reperfusion injury revealed differential expression of
caspases with marked increase in CPP32 and ICE mRNA and proteins during
reperfusion, transient increase in Nedd2 mRNA and proteins during
ischemia and the early period of reperfusion, and little change
in Mch2 expression during the ischemia or reperfusion period.
The altered expression suggests that caspases may act in concert in a
cascade and may play an important role in ischemic acute renal failure.
gene expression; interleukin-1-converting enzyme; CPP32; Nedd2; Mch2; cysteine proteases
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