| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Renal Division, Vanderbilt University Medical Center, Nashville, Tennessee 37232-2372; and 2 Endocrine-Hypertension Unit, Department of Medicine, Brigham & Women's Hospital Harvard Medical School, Boston, Massachusetts 02115
We previously identified transcripts encoding a
G protein-coupled, extracellular calcium/polyvalent cation-sensing
receptor, RaKCaR, in rat kidney (D. Riccardi, J. Park, W.-S. Lee, G. Gamba, E. M. Brown, and S. C. Hebert. Proc. Natl.
Acad. Sci. USA 92: 131-135, 1994), which was
proposed to provide the mechanism for modulating a variety of renal
functions in response to changes in extracellular
Ca2+ (E. M. Brown. In:
Handbook of Physiology. Bethesda, MD:
Am. Physiol. Soc., 1992, sect. 8, vol. 2, chapt. 39, p. 1841-1916;
and S. C. Hebert. Kidney Int. 50:
2129-2139, 1996). Here, we examine the cellular and regional
distribution of receptor protein by immunofluorescence microscopy using
a polyclonal antibody raised against a 22 amino acid region of the
NH2 terminus of the receptor. The
most intense fluorescence was seen at the basolateral border of
cortical thick ascending limb cells. Basolateral staining for the
receptor was also detected in medullary thick ascending limbs, in
macula densa cells identified by costaining with antibody to brain
nitric oxide synthase, NOS-B1, and in distal convoluted tubule cells
distinguished by costaining for the apical thiazide-sensitive
Na+-Cl
cotransporter. Apical anti-RaKCaR staining was detected at the base of
the brush border of proximal tubules with decreasing intensity from S1
to S3 segments. In cortical collecting ducts, anti-RaKCaR staining was
detected in some, but not all, type A intercalated cells identified by
costaining with anti-H+-ATPase and
anti-AE1
Cl/
exchanger antibodies. The present study demonstrates that RaKCaR
protein is expressed in many different nephron segments and that the
polarity of receptor expression varies with cell type along the
nephron. These results suggest potential roles for the extracellular
Ca2+/polyvalent cation-sensing
receptor in responding to both circulating and urinary concentrations
of divalent minerals and potentially other polyvalent cations (e.g.,
aminoglycoside antibiotics) to modulate nephron function.
calcium-sensing receptor; G protein-coupled receptor; immunofluorescence; macula densa; nitric oxide synthase; AE1 anion exchanger; chloride/bicarbonate exchanger; proton-adenosinetriphosphatase; thiazide-sensitive sodium-chloride cotransporter; nitric oxide synthase; kidney tubules
This article has been cited by other articles:
|
|
 |
|
  H. I. Abdullah, P. L. Pedraza, J. C. McGiff, and N. R. Ferreri Calcium-sensing receptor signaling pathways in medullary thick ascending limb cells mediate COX-2-derived PGE2 production: functional significance Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1082 - F1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. I. Abdullah, P. L. Pedraza, J. C. McGiff, and N. R. Ferreri CaR activation increases TNF production by mTAL cells via a Gi-dependent mechanism Am J Physiol Renal Physiol, February 1, 2008; 294(2): F345 - F354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Molostvov, S. James, S. Fletcher, J. Bennett, H. Lehnert, R. Bland, and D. Zehnder Extracellular calcium-sensing receptor is functionally expressed in human artery Am J Physiol Renal Physiol, September 1, 2007; 293(3): F946 - F955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Huang, A. Sindic, C. E. Hill, K. M. Hujer, K. W. Chan, M. Sassen, Z. Wu, Y. Kurachi, S. Nielsen, M. F. Romero, et al. Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function Am J Physiol Renal Physiol, March 1, 2007; 292(3): F1073 - F1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chattopadhyay, K.-H. Jeong, S. Yano, S. Huang, J. L. Pang, X. Ren, E. Terwilliger, U. B. Kaiser, P. M. Vassilev, M. R. Pollak, et al. Calcium receptor stimulates chemotaxis and secretion of MCP-1 in GnRH neurons in vitro: potential impact on reduced GnRH neuron population in CaR-null mice Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E523 - E532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Kempson, J. M. Edwards, and M. Sturek Inhibition of the renal betaine transporter by calcium ions Am J Physiol Renal Physiol, August 1, 2006; 291(2): F305 - F313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. I. Abdullah, P. L. Pedraza, S. Hao, K. D. Rodland, J. C. McGiff, and N. R. Ferreri NFAT regulates calcium-sensing receptor-mediated TNF production Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1110 - F1117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Ward, D. Maldonado-Perez, L. Hollins, and D. Riccardi Aminoglycosides Induce Acute Cell Signaling and Chronic Cell Death in Renal Cells that Express the Calcium-Sensing Receptor J. Am. Soc. Nephrol., May 1, 2005; 16(5): 1236 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gamba Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters Physiol Rev, April 1, 2005; 85(2): 423 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels Calcium Absorption Across Epithelia Physiol Rev, January 1, 2005; 85(1): 373 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, C. Li, T.-H. Kwon, R. T. Miller, M. A. Knepper, J. Frokiaer, and S. Nielsen Reduced expression of renal Na+ transporters in rats with PTH-induced hypercalcemia Am J Physiol Renal Physiol, March 1, 2004; 286(3): F534 - F545. [Abstract] [Full Text]
|
|
|
|
|
|
J. Ba, D. Brown, and P. A. Friedman Calcium-sensing receptor regulation of PTH-inhibitable proximal tubule phosphate transport Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1233 - F1243. [Abstract] [Full Text]
|
|
|
|
|
|
H. Hentschel, J. Nearing, H. W. Harris, M. Betka, M. Baum, S. C. Hebert, and M. Elger Localization of Mg2+-sensing shark kidney calcium receptor SKCaR in kidney of spiny dogfish, Squalus acanthias Am J Physiol Renal Physiol, September 1, 2003; 285(3): F430 - F439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang, P. L. Pedraza, H. I. Abdullah, J. C. McGiff, and N. R. Ferreri Calcium-sensing receptor-mediated TNF production in medullary thick ascending limb cells Am J Physiol Renal Physiol, November 1, 2002; 283(5): F963 - F970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. I. Motoyama and P. A. Friedman Calcium-sensing receptor regulation of PTH-dependent calcium absorption by mouse cortical ascending limbs Am J Physiol Renal Physiol, September 1, 2002; 283(3): F399 - F406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. X. Cheng, M. Okuda, A. E. Hall, J. P. Geibel, and S. C. Hebert Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G240 - G250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang, S.-J. An, W.-H. Wang, J. C. McGiff, and N. R. Ferreri CaR-mediated COX-2 expression in primary cultured mTAL cells Am J Physiol Renal Physiol, October 1, 2001; 281(4): F658 - F664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, T.-H. Kwon, C. Li, A. Flyvbjerg, M. A. Knepper, J. Frokiar, and S. Nielsen Altered expression of renal aquaporins and Na+ transporters in rats treated with L-type calcium blocker Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1632 - R1641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Ferguson, M. Wareing, D. T. Ward, R. Green, C. P. Smith, and D. Riccardi Cellular localization of divalent metal transporter DMT-1 in rat kidney Am J Physiol Renal Physiol, May 1, 2001; 280(5): F803 - F814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Dai, G. Ritchie, D. Kerstan, H. S. Kang, D. E. C. Cole, and G. A. Quamme Magnesium Transport in the Renal Distal Convoluted Tubule Physiol Rev, January 1, 2001; 81(1): 51 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Brown and R. J. MacLeod Extracellular Calcium Sensing and Extracellular Calcium Signaling Physiol Rev, January 1, 2001; 81(1): 239 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Murer, N. Hernando, I. Forster, and J. Biber Proximal Tubular Phosphate Reabsorption: Molecular Mechanisms Physiol Rev, October 1, 2000; 80(4): 1373 - 1409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chattopadhyay, C. Evliyaoglu, O. Heese, R. Carroll, J. Sanders, P. Black, and E. M. Brown Regulation of secretion of PTHrP by Ca2+-sensing receptor in human astrocytes, astrocytomas, and meningiomas Am J Physiol Cell Physiol, September 1, 2000; 279(3): C691 - C699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Helies-Toussaint, L. Aarab, J.-M. Gasc, J.-M. Verbavatz, and D. Chabardes Cellular localization of type 5 and type 6 ACs in collecting duct and regulation of cAMP synthesis Am J Physiol Renal Physiol, July 1, 2000; 279(1): F185 - F194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Bapty, L.-J. Dai, G. Ritchie, L. Canaff, G. N. Hendy, and G. A. Quamme Mg2+/Ca2+ sensing inhibits hormone-stimulated Mg2+ uptake in mouse distal convoluted tubule cells Am J Physiol Renal Physiol, September 1, 1998; 275(3): F353 - F360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Young and E. Rozengurt Amino acids and Ca2+ stimulate different patterns of Ca2+ oscillations through the Ca2+-sensing receptor Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1414 - C1422. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
