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reabsorption in OMCD by
activation of colonic
H+-K+-ATPase
Department of Medicine, University of Cincinnati School of Medicine, and Veterans Affairs Medical Center, Cincinnati, Ohio 45267
To probe the role of the isoforms of
H+-K+-ATPase
(HKA) in potassium depletion (KD), rats were placed on a KD diet for 2 wk. Colonic HKA (cHKA) mRNA levels increased ~30-fold in outer
medulla, and net 
flux
(JtCO2)
in outer medullary collecting duct (OMCD) increased (13.1 pmol · min
1 · mm
tubule length1 in control
to 17.7 pmol · min1 · mm
tubule length1 in KD;
P < 0.01). In normal rats, 1 mM
ouabain in perfusate had no effect on
JtCO2,
whereas 10 µM Sch-28080 decreased JtCO2
to 5.1 pmol · min1 · mm
tubule length1
(P < 0.001). In KD rats, ouabain 1 mM decreased
JtCO2 to 6.3 pmol · min1 · mm
tubule length1
(P < 0.001). Although 10 µM
Sch-28080 also decreased
JtCO2 to 4.6 pmol · min1 · mm
tubule length1
(P < 0.001), the inhibitory effects
of Sch-28080 and ouabain were not additive. Removal of
K+ from perfusate blocked
Sch-28080-sensitive
JtCO2
in both normal and KD tubules. The data suggest that, in KD, cHKA is
induced and mediates increased
reabsorption in OMCD, cHKA in vivo is sensitive to both Sch-28080 and
ouabain, and cHKA activity is dominant.
acid-base homeostasis; alkalosis; proton secretion; potassium reabsorption; hydrogen-potassium-adenosinetriphosphatase; outer medullary collecting duct
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