AJP - Renal AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 274: F783-F790, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kurasaki, M.
Right arrow Articles by Suzuki-Kurasaki, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kurasaki, M.
Right arrow Articles by Suzuki-Kurasaki, M.
Vol. 274, Issue 4, F783-F790, April 1998

Copper metabolism in the kidney of rats administered copper and copper-metallothionein

Masaaki Kurasaki, Masashi Okabe, Shigeru Saito, and Mika Suzuki-Kurasaki

Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan

To gain a greater understanding of the mechanism of Cu metabolism in kidneys of rats, using autofluorescence of Cu-metallothioneins (Cu-MTs) we revealed the behavior of Cu-MT in the kidneys of rats administered Cu-MT. Yellow and orange fluorescent signals of Cu-MT were observed in the cortex. By microscopic studies, Cu-MT was dominant in the proximal convolute tubular cells of the cortex. A high concentration of Cu-MT presented in the lysosome-like organelles of the proximal convolute tubular adjacent to the glomeruli. During the time course after the injection, the orange signal in lysosome-like organelles gradually converted to a yellow signal, indicating that the Cu-MT was involved in a degradation process in lysosomes by oxidation, and the MT mRNA increased in the cortex, although the immunoreactivity of MT was almost constant in the same region. These results suggested that Cu bound to the injected MT was released in lysosomes and became a new inducer of MT biosynthesis in the cortex. In conclusion, the biosynthesis and degradation of Cu-MT occur repeatedly in the proximal convolute tubular cells.

autofluorescence; in situ blotting


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Linz, N. L. Barnes, A. M. Zimnicka, J. H. Kaplan, B. Eipper, and S. Lutsenko
Intracellular targeting of copper-transporting ATPase ATP7A in a normal and Atp7b / kidney
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F53 - F61.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Saito, M. Kurasaki, T. Hosokawa, M. Okabe, T. Saito, Y. Fujii, K. Nagashima, and K. Yoshida
Localization of the Induced Metallothionein and DNA Damage in Rat Kidney After Gold Injection
J. Histochem. Cytochem., September 1, 2002; 50(9): 1263 - 1271.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online