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Am J Physiol Renal Physiol 274: F834-F840, 1998;
0363-6127/98 $5.00
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Vol. 274, Issue 5, F834-F840, May 1998

Dose-dependent effects of CRF-like diuretic peptide on transcellular and paracellular transport pathways

Thomas M. Clark1, Timothy K. Hayes2, and Klaus W. Beyenbach3

1 Department of Zoology, Washington State University, Pullman, Washington 99164; 2 Bayer Corporation, Raleigh, North Carolina 27606; and 3 Section of Physiology, Cornell University, Ithaca, New York 14853

The mechanism of action of synthetic Culex corticotropin-releasing factor (CRF)-like diuretic peptide (CCRF-DP) was investigated in isolated, perfused Malpighian tubules of the yellow fever mosquito, Aedes aegypti. Low concentrations of CCRF-DP (10-10 and 10-9 M) caused depolarizing oscillations of the lumen-positive transepithelial voltage (Vt) in Malpighian tubules, whereas high concentrations (10-8 and 10-7 M) first depolarized and then transiently hyperpolarized Vt; CCRF-DP always lowered transepithelial resistance (Rt), regardless of voltage depolarization or hyperpolarization. The short-circuit current (Isc), an electrical estimate of active transepithelial transport of Na and K, remained unchanged at low concentrations of CCRF-DP, but Isc more than doubled at high concentrations. These effects of CCRF-DP suggest dose-dependent sites of action: low concentrations of CCRF-DP affect the paracellular pathway, and high concentrations affect both paracellular and transcellular pathways.

Aedes aegypti; transepithelial voltage; transepithelial resistance; short-circuit current; regulation of shunt pathway


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