AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 274: F1029-F1036, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gaudio, K. M.
Right arrow Articles by Siegel, N. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaudio, K. M.
Right arrow Articles by Siegel, N. J.
Vol. 274, Issue 6, F1029-F1036, June 1998

Role of heat stress response in the tolerance of immature renal tubules to anoxia

Karen M. Gaudio, Gunilla Thulin, Andrea Mann, Michael Kashgarian, and Norman J. Siegel

Departments of Pediatrics and Pathology, Yale University School of Medicine, New Haven, Connecticut 06520

The stress response was studied in suspensions of tubules from immature (IT) and mature (MT) rats after noninjury, heat, oxygen, and anoxia. Under all conditions, IT exhibited more exuberant activation of heat shock transcription factor (HSF) than MT. Characterization of activated HSF in immature cortex revealed HSF1. Also, 2 h after each condition, heat shock protein-72 (HSP-72) mRNA was twofold in IT. As the metabolic response to 45 min of anoxia, 20-min reoxygenation was assessed by measuring O2 consumption (O2C). Basal O2C was manipulated with ouabain, nystatin, and carbonylcyanide p-chloromethyoxyphenylhydrazone (CCCP). Basal O2C in IT were one-half the value of MT. After anoxia, basal O2C was reduced by a greater degree in MT. Ouabain reduced O2C to half the basal value in both noninjured and anoxic groups. Basal O2C was significantly stimulated by nystatin but not to the same level following anoxia in MT and IT. Basal O2C was also stimulated by CCCP, but after anoxia, CCCP O2C was significantly less in MT with no decrease in IT, suggesting mitochondria are better preserved in IT. Also, O2C devoted to nontransport activity was better maintained in IT.

heat stress protein-72; activated heat shock transcription factor; oxygen consumption rate; immature proximal renal tubules


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Lasaitiene, Y. Chen, V. Mildaziene, Z. Nauciene, B. Sundelin, B. R. Johansson, M. Yano, and P. Friberg
Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1260 - F1269.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Sreedharan, M. Riordan, S. Wang, G. Thulin, M. Kashgarian, and N. J. Siegel
Reduced tolerance of immature renal tubules to anoxia by HSF-1 decoy
Am J Physiol Renal Physiol, February 1, 2005; 288(2): F322 - F326.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F.-X. Beck, W. Neuhofer, and E. Muller
Molecular chaperones in the kidney: distribution, putative roles, and regulation
Am J Physiol Renal Physiol, August 1, 2000; 279(2): F203 - F215.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online