AJP - Renal AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 275: F103-F110, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohaupt, M. G.
Right arrow Articles by Schulze-Lohoff, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mohaupt, M. G.
Right arrow Articles by Schulze-Lohoff, E.
Vol. 275, Issue 1, F103-F110, July 1998

Activation of purinergic P2Y2 receptors inhibits inducible NO synthase in cultured rat mesangial cells

Markus G. Mohaupt, Tina Fischer, Jörg Schwöbel, R. Bernd Sterzel, and Eckhard Schulze-Lohoff

Nephrologisches Labor, Medizinische Klinik IV, Universität Erlangen-Nürnberg, 91054 Erlangen, Germany

Cytokine-induced nitric oxide (NO) is produced on glomerular inflammation. Glomerular injury and thrombocyte aggregation result in the release of nucleotides, which may regulate induced NO synthesis in cultured rat mesangial cells (MCs). ATP (10-3 M) inhibited 24-h nitrite production induced by lipopolysaccharide (LPS, 10 µg/ml)/interferon-gamma (IFN-gamma , 100 U/ml) by 48.2 ± 6.3%, as well as induction of inducible NOS (iNOS) protein and mRNA. Also, coincubation with either 10-4 M of UTP, ATP, or ATPgamma S inhibited LPS/IFN-gamma -induced nitrite production by 29.9 ± 5.8, 36.4 ± 4.3, and 50.3 ± 6.5%, respectively, indicating involvement of purinergic P2Y2 receptors. Correspondingly, cultured MCs expressed P2Y2 receptor mRNA. Agonists for other purinergic receptors [alpha ,beta -methylene-ATP, 3'-O-(4-benzoyl)-benzoyl-ATP, 2-methylthio-ATP, ADP, UDP, adenosine] were ineffective. Treatment with the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA, 10-8 M) reproduced the inhibitory effect of ATP on iNOS protein expression and nitrite inhibition (by 46.6 ± 10.4%). The effect of ATP or PMA was reversed by the PKC inhibitors Ro-31-8220 (10-8 M) and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (10-5 M), indicating that suppression of iNOS is mediated via activation of PKC through stimulated P2Y2 receptors. In conclusion, the release of purine mediators may play a critical role for iNOS expression and synthesis of NO during glomerular inflammatory disorders.

purinergic receptors; inducible nitric oxide synthesis; glomerular inflammation; protein kinase C


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Rivera, S. Zhang, B. S. Fuller, B. Edwards, T. Seki, M.-H. Wang, M. B. Marrero, and E. W. Inscho
P2 receptor regulation of [Ca2+]i in cultured mouse mesangial cells
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1380 - F1389.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Liu, A. M. Gutierrez, A. Ring, and A. E. G. Persson
Nitric Oxide Induces Resensitization of P2Y Nucleotide Receptors in Cultured Rat Mesangial Cells
J. Am. Soc. Nephrol., February 1, 2002; 13(2): 313 - 321.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
C.-B. Lanz, M. Causevic, C. Heiniger, F. J. Frey, B. M. Frey, and M. G. Mohaupt
Fluid Shear Stress Reduces 11{beta}-Hydroxysteroid Dehydrogenase Type 2
Hypertension, January 1, 2001; 37(1): 160 - 169.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Schwobel, T. Fischer, B. Lanz, and M. Mohaupt
Angiotensin II receptor subtypes determine induced NO production in rat glomerular mesangial cells
Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1092 - F1100.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Hou, S. Moller, L. Edvinsson, and D. Erlinge
Cytokines Induce Upregulation of Vascular P2Y2 Receptors and Increased Mitogenic Responses to UTP and ATP
Arterioscler Thromb Vasc Biol, September 1, 2000; 20(9): 2064 - 2069.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. Umino, E. Kusano, S. Muto, T. Akimoto, S. Yanagiba, S. Ono, M. Amemiya, Y. Ando, S. Homma, U. Ikeda, et al.
AVP inhibits LPS- and IL-1beta -stimulated NO and cGMP via V1 receptor in cultured rat mesangial cells
Am J Physiol Renal Physiol, March 1, 1999; 276(3): F433 - F441.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online