| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Medicine,
To understand better
the function of endothelin-1 (ET-1) in renal physiology, we examined
vascular and glomerular expression of ET-1 in normal human kidney and
in lupus nephritis. Immunohistochemical analysis revealed that renal
endothelium of glomeruli, arteries, veins, and capillaries expressed
ET-1. Endothelial cells were the principal source of glomerular ET-1;
positive immunostaining was detected only rarely in mesangial cells and
vascular smooth muscle cells from normal kidney. However, mesangial
staining for ET-1 was elevated in patients with lupus nephritis,
suggesting that under certain conditions mesangial cells elaborate
ET-1. Indeed cultured human mesangial cells from normal
subjects secreted ET-1 peptide. ET-1 secretion was augmented by the
protein kinase C activator phorbol ester and by transforming growth
factor-
1 (TGF-1), a cytokine implicated in the development of
glomerulosclerosis. Transient transfection of cultured mesangial cells
with a preproET-1 reporter construct showed that the preproET-1
promoter is transcriptionally active in mesangial cells and is
stimulated by TGF-1, phorbol ester, or ectopic expression of protein
kinase 1. Cultured human mesangial cells have both
ETA and
ETB receptors that contribute to
ET-1-stimulated mitogenesis. Taken together, these results demonstrate
that ET-1 is expressed at sites where paracrine or autocrine signaling
by ET-1 might control renal vasoconstriction, glomerular filtration
rate, and remodeling of the glomerulus in renal disease.
vasoactive peptides; G protein-coupled receptors; renal hemodynamics
This article has been cited by other articles:
|
|
 |
|
  R. Mishra, P. Leahy, and M. S. Simonson Gene expression profile of endothelin-1-induced growth in glomerular mesangial cells Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1109 - C1115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sorokin and D. E. Kohan Physiology and pathology of endothelin-1 in renal mesangium Am J Physiol Renal Physiol, October 1, 2003; 285(4): F579 - F589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mishra, P. Leahy, and M. S. Simonson Gene expression profiling reveals role for EGF-family ligands in mesangial cell proliferation Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1151 - F1159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. LEHRKE, R. WALDHERR, E. RITZ, and J. WAGNER Renal Endothelin-1 and Endothelin Receptor Type B Expression in Glomerular Diseases with Proteinuria J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2321 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. FOSCHI, A. SOROKIN, P. PRATT, A. MCGINTY, G. L. VILLA, F. FRANCHI, and M. J. DUNN PreproEndothelin-1 Expression in Human Mesangial Cells: Evidence for a p38 Mitogen-Activated Protein Kinase/Protein Kinases-C--Dependent Mechanism J. Am. Soc. Nephrol., June 1, 2001; 12(6): 1137 - 1150. [Abstract] [Full Text]
|
|
|
|
|
|
P. INIGO, J. M. CAMPISTOL, S. LARIO, C. PIERA, B. CAMPOS, M. BESCOS, F. OPPENHEIMER, and F. RIVERA Effects of Losartan and Amlodipine on Intrarenal Hemodynamics and TGF-{beta}1 Plasma Levels in a Crossover Trial in Renal Transplant Recipients J. Am. Soc. Nephrol., April 1, 2001; 12(4): 822 - 827. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
