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1 Nephrology and Hypertension
Services,
This study
explores the role of K+ and
aldosterone in the regulation of mRNA of the
ATP-sensitive, inwardly rectifying
K+ channel, ROMK, in the rat
kidney. K+ deficiency
downregulated ROMK mRNA in cortex to 47.1 ± 5.1% of control (P < 0.001) and in medulla
to 56.1 ± 3.4% (P < 0.001). High-K+ diet slightly increased
ROMK mRNA in medulla to 122 ± 9%
(P < 0.05 vs. control).
Adrenalectomy (Adx) downregulated cortical ROMK mRNA to 30.7 ± 6.8% (P < 0.001 vs. control), and
increased it in medulla to 138 ± 12.9%
(P < 0.02 vs. control). In Adx rats, K+ deficiency decreased ROMK mRNA
in cortex and medulla similar to intact rats. The
1- and
1-Na-K-ATPase subunits were
regulated in parallel to that of ROMK. In medulla, ROMK mRNA correlated with serum K+ concentration at
R = 0.9406 (n = 6, P < 0.001) and
1-Na-K-ATPase mRNA at
R = 0.9756 (n = 6, P < 0.001). ROMK2 also correlated
with serum K+ concentration
(R = 0.895;
n = 6, P < 0.01). These results
show that cortical ROMK expression is regulated by aldosterone and K+, whereas the medullary ROMK
mRNA is regulated by serum K+.
medullary thick ascending limb; cortical collecting duct; adrenalectomy
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