| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Internal Medicine, Veterans Affairs Medical Center, Louisville 40206; and Department of Internal Medicine, University of Louisville, Louisville, Kentucky 40202-1718
The purpose of the present study was to determine the effect of protein kinase A and protein kinase C activation on the membrane expression of NaPi-4, the type II sodium-phosphate cotransporter in OK cells. NaPi-4 expression was measured using polyclonal antisera produced in rabbits against a peptide identical to the carboxy-terminal 12-amino acid sequence of NaPi-4. The antisera identified an apically localized protein by confocal imaging of intact OK cells and a broad band of 110-140 kDa by immunoblot analysis of OK cell membranes. Treatment of OK cells with parathyroid hormone (PTH) decreased the intensity of the 110- to 140-kDa band, which was detectable by 2 h, maximal by 4 h at 62%, and sustained for 24 h. 8-Bromo-cAMP (8-BrcAMP) inhibited NaPi-4 expression for up to 24 h by over 90%. However, phorbol 12-myristate 13-acetate inhibited NaPi-4 expression by less than 10%. PTH-(3-34), a fragment which stimulates only protein kinase C, inhibited phosphate transport but also had no effect on NaPi-4 expression. We conclude that protein kinase A but not protein kinase C inhibits sodium-phosphate uptake in OK cells by downregulation of NaPi-4 expression.
opossum kidney cells; parathyroid hormone; type II sodium-phosphate cotransporter; confocal imaging; phorbol 12-myristate 13-acetate; 8-bromoadenosine 3',5'-cyclic monophosphate
This article has been cited by other articles:
|
|
 |
|
  M. Wieser, G. Stadler, P. Jennings, B. Streubel, W. Pfaller, P. Ambros, C. Riedl, H. Katinger, J. Grillari, and R. Grillari-Voglauer hTERT alone immortalizes epithelial cells of renal proximal tubules without changing their functional characteristics Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1365 - F1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Khundmiri, A. Ahmad, R. E. Bennett, E. J. Weinman, D. Steplock, J. Cole, P. D. Baumann, J. Lewis, S. Singh, B. J. Clark, et al. Novel regulatory function for NHERF-1 in Npt2a transcription Am J Physiol Renal Physiol, April 1, 2008; 294(4): F840 - F849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Lanaspa, H. Giral, S. Y. Breusegem, N. Halaihel, G. Baile, J. Catalan, J. A. Carrodeguas, N. P. Barry, M. Levi, and V. Sorribas Interaction of MAP17 with NHERF3/4 induces translocation of the renal Na/Pi IIa transporter to the trans-Golgi Am J Physiol Renal Physiol, January 1, 2007; 292(1): F230 - F242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Deliot, N. Hernando, Z. Horst-Liu, S. M. Gisler, P. Capuano, C. A. Wagner, D. Bacic, S. O'Brien, J. Biber, and H. Murer Parathyroid hormone treatment induces dissociation of type IIa Na+-Pi cotransporter-Na+/H+ exchanger regulatory factor-1 complexes Am J Physiol Cell Physiol, July 1, 2005; 289(1): C159 - C167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Breusegem, N. Halaihel, M. Inoue, H. Zajicek, E. Lederer, N. P. Barry, V. Sorribas, and M. Levi Acute and chronic changes in cholesterol modulate Na-Pi cotransport activity in OK cells Am J Physiol Renal Physiol, July 1, 2005; 289(1): F154 - F165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pribanic, S. M. Gisler, D. Bacic, C. Madjdpour, N. Hernando, V. Sorribas, A. Gantenbein, J. Biber, and H. Murer Interactions of MAP17 with the NaPi-IIa/PDZK1 protein complex in renal proximal tubular cells Am J Physiol Renal Physiol, October 1, 2003; 285(4): F784 - F791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Blasco, J. J. Aramayona, A. I. Alcalde, J. Catalan, M. Sarasa, and V. Sorribas Rat kidney MAP17 induces cotransport of Na-mannose and Na-glucose in Xenopus laevis oocytes Am J Physiol Renal Physiol, October 1, 2003; 285(4): F799 - F810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Karim-Jimenez, N. Hernando, J. Biber, and H. Murer A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter PNAS, October 23, 2000; (2000) 220394197. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Murer, N. Hernando, I. Forster, and J. Biber Proximal Tubular Phosphate Reabsorption: Molecular Mechanisms Physiol Rev, October 1, 2000; 80(4): 1373 - 1409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Traebert, H. Volkl, J. Biber, H. Murer, and B. Kaissling Luminal and contraluminal action of 1-34 and 3-34 PTH peptides on renal type IIa Na-Pi cotransporter Am J Physiol Renal Physiol, May 1, 2000; 278(5): F792 - F798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. LEDERER, S. S. SOHI, and K. R. MCLEISH Parathyroid Hormone Stimulates Extracellular Signal-Regulated Kinase (ERK) Activity Through Two Independent Signal Transduction Pathways: Roleof ERK in Sodium-Phosphate Cotransport J. Am. Soc. Nephrol., February 1, 2000; 11(2): 222 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Fernandes, R. Beliveau, G. Friedlander, and C. Silve NaPO4 cotransport type III (PiT1) expression in human embryonic kidney cells and regulation by PTH Am J Physiol Renal Physiol, October 1, 1999; 277(4): F543 - F551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. YOSHIDA, N. YOSHIDA, A. NAKAMURA, T. MONKAWA, M. HAYASHI, and T. SARUTA Cloning of Porcine 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase and Its Regulation by cAMP in LLC-PK1 Cells J. Am. Soc. Nephrol., May 1, 1999; 10(5): 963 - 970. [Abstract] [Full Text]
|
|
|
|
|
|
M. F. Pfister, J. Forgo, U. Ziegler, J. Biber, and H. Murer cAMP-dependent and -independent downregulation of type II Na-Pi cotransporters by PTH Am J Physiol Renal Physiol, May 1, 1999; 276(5): F720 - F725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Karim-Jimenez, N. Hernando, J. Biber, and H. Murer A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter PNAS, November 7, 2000; 97(23): 12896 - 12901. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
