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Am J Physiol Renal Physiol 275: F361-F369, 1998;
0363-6127/98 $5.00
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Vol. 275, Issue 3, F361-F369, September 1998

PMA and staurosporine affect expression of the PCK gene in LLC-PK1-F+ cells

Wenlin Liu1, Elisabeth Feifel2, Thomas Holcomb1, Xiangdong Liu1, Nikolaus Spitaler2, Gerhard Gstraunthaler2, and Norman P. Curthoys1

1 Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870; and 2 Institute of Physiology, University of Innsbruck, A-6010 Innsbruck, Austria

The addition of phorbol 12-myristate 13-acetate (PMA) to renal LLC-PK1-F+ cells caused a rapid decrease in the level of phosphoenolpyruvate carboxykinase (PCK) mRNA and reversed the stimulatory effects of exposure to acidic medium (pH 6.9, 10 mM HCO-3) or cAMP. In contrast, prolonged treatment with PMA increased the levels of PCK mRNA. The two effects correlated with the membrane translocation and downregulation of the alpha -isozyme of protein kinase C and were blocked by pretreatment with specific inhibitors of protein kinase C. The rapid decrease in PCK mRNA caused by PMA occurred with a half-life (t1/2 = 1 h) that is significantly faster than that measured during recovery from acid medium or following inhibition of transcription (t1/2 = 4 h). The effect of PMA was reversed by staurosporine, which apparently acts by inhibiting a signaling pathway other than protein kinase C. Staurosporine had no effect on the half-life of the PCK mRNA, but it stimulated the activity of a chloramphenicol acetyltransferase gene that was driven by the initial 490 base pairs of the PCK promoter and transiently transfected into LLC-PK1-F+ cells. This effect was additive to that of cAMP, and neither stimulation was reversed by PMA. The stimulatory effect of staurosporine was mapped to the cAMP response element (CRE-1) and P3(II) element of the PCK promoter. The data indicate that, in LLC-PK1-F+ cells, activation of protein kinase C decreases the stability of the PCK mRNA, whereas transcription of the PCK gene may be suppressed by a kinase that is inhibited by staurosporine.

protein kinase C; transcription; messenger ribonucleic acid stability; proximal tubule; phosphoenolpyruvate carboxykinase; phorbol 12-myristate 13-acetate


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