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Am J Physiol Renal Physiol 275: F802-F811, 1998;
0363-6127/98 $5.00
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Vol. 275, Issue 5, F802-F811, November 1998

Morphological and functional characterization of a conditionally immortalized collecting tubule cell line

Marcia Takacs-Jarrett, William E. Sweeney, Ellis D. Avner, and Calvin U. Cotton

Departments of Pediatrics, and Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106-4948

A conditionally immortalized collecting tubule cell line, mCT1, was derived from the H-2Kb-ts A58 transgenic mouse (ImmortoMouse), which harbors a temperature-sensitive mutant of the SV40 large T antigen oncogene. Cells maintained under permissive conditions [33°C with interferon-gamma (IFN-gamma )] form epithelial monolayers, express large T antigen, and proliferate (>50 passages). The cells retain properties characteristic of the renal collecting tubule (CT) including: vasopressin (VP)-stimulated cAMP accumulation, aquaporin-2 expression, high transepithelial electrical resistance, VP-stimulated ion transport, and amiloride-sensitive sodium absorption. When the cells are transferred to nonpermissive conditions (39°C without IFN-gamma ), the steady-state level of large T antigen protein declines (>95% decrease) and cell proliferation is arrested. This conditionally immortalized, murine renal cell line should prove useful for studies of CT physiology and large T antigen biology.

SV40 large T antigen; temperature sensitivity; chloride secretion; sodium absorption; amiloride; vasopressin; aquaporin-2


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