AJP - Renal Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 275: F802-F811, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takacs-Jarrett, M.
Right arrow Articles by Cotton, C. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takacs-Jarrett, M.
Right arrow Articles by Cotton, C. U.
Vol. 275, Issue 5, F802-F811, November 1998

Morphological and functional characterization of a conditionally immortalized collecting tubule cell line

Marcia Takacs-Jarrett, William E. Sweeney, Ellis D. Avner, and Calvin U. Cotton

Departments of Pediatrics, and Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106-4948

A conditionally immortalized collecting tubule cell line, mCT1, was derived from the H-2Kb-ts A58 transgenic mouse (ImmortoMouse), which harbors a temperature-sensitive mutant of the SV40 large T antigen oncogene. Cells maintained under permissive conditions [33°C with interferon-gamma (IFN-gamma )] form epithelial monolayers, express large T antigen, and proliferate (>50 passages). The cells retain properties characteristic of the renal collecting tubule (CT) including: vasopressin (VP)-stimulated cAMP accumulation, aquaporin-2 expression, high transepithelial electrical resistance, VP-stimulated ion transport, and amiloride-sensitive sodium absorption. When the cells are transferred to nonpermissive conditions (39°C without IFN-gamma ), the steady-state level of large T antigen protein declines (>95% decrease) and cell proliferation is arrested. This conditionally immortalized, murine renal cell line should prove useful for studies of CT physiology and large T antigen biology.

SV40 large T antigen; temperature sensitivity; chloride secretion; sodium absorption; amiloride; vasopressin; aquaporin-2


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Falin, I. E. Veizis, and C. U. Cotton
A role for ERK1/2 in EGF- and ATP-dependent regulation of amiloride-sensitive sodium absorption
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1003 - C1011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. I. Veizis, C. R. Carlin, and C. U. Cotton
Decreased amiloride-sensitive Na+ absorption in collecting duct principal cells isolated from BPK ARPKD mice
Am J Physiol Renal Physiol, February 1, 2004; 286(2): F244 - F254.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Sinha, Z. Wang, V. R. Price, J. H. Schwartz, and W. Lieberthal
Chemical anoxia of tubular cells induces activation of c-Src and its translocation to the zonula adherens
Am J Physiol Renal Physiol, March 1, 2003; 284(3): F488 - F497.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J.-P. Shen and C. U. Cotton
Epidermal growth factor inhibits amiloride-sensitive sodium absorption in renal collecting duct cells
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F57 - F64.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. K. Yoder, A. Tousson, L. Millican, J. H. Wu, C. E. Bugg Jr., J. A. Schafer, and D. F. Balkovetz
Polaris, a protein disrupted in orpk mutant mice, is required for assembly of renal cilium
Am J Physiol Renal Physiol, March 1, 2002; 282(3): F541 - F552.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. E. Sweeney Jr., L. Kusner, C. R. Carlin, S. Chang, L. Futey, C. U. Cotton, K. M. Dell, and E. D. Avner
Phenotypic analysis of conditionally immortalized cells isolated from the BPK model of ARPKD
Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1695 - C1705.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Takacs-Jarrett, W. E. Sweeney, E. D. Avner, and C. U. Cotton
Generation and phenotype of cell lines derived from CF and non-CF mice that carry the H-2Kb-tsA58 transgene
Am J Physiol Cell Physiol, January 1, 2001; 280(1): C228 - C236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online