AJP - Renal AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 275: F840-F847, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frick, K. K.
Right arrow Articles by Bushinsky, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frick, K. K.
Right arrow Articles by Bushinsky, D. A.
Vol. 275, Issue 5, F840-F847, November 1998

Chronic metabolic acidosis reversibly inhibits extracellular matrix gene expression in mouse osteoblasts

Kevin K. Frick and David A. Bushinsky

Nephrology Unit, Department of Medicine, University of Rochester School of Medicine, Rochester, New York 14642

Chronic metabolic acidosis induces net calcium efflux from bone mineral through an increase in osteoclastic resorption and a decrease in osteoblastic matrix deposition and mineralization. To determine the effects of chronic metabolic acidosis on the expression of genes necessary for mineralization, we grew primary bone cells, which are principally osteoblasts, to confluence in neutral pH (7.5) medium and then switched the cells either to a neutral pH or to an acidic pH (7.1) differentiation medium. Cells were harvested for RNA at 4- to 7-day intervals for up to 44 days. By 36 days, there was extensive bone nodule formation and mineralization in cells cultured in neutral medium; however, there was a substantial decrease in nodule formation and mineralization in cells cultured in acidic medium. There was a marked increase in matrix Gla protein RNA and an increase in osteopontin RNA in neutral cultures; however, acidic medium almost completely prevented any increase. In contrast, RNA levels for osteonectin and transforming growth factor-beta 1 were not altered by chronic acidosis. Additional cells were incubated in acid differentiation medium for 1, 2, or 3 wk and then transferred to neutral medium; in each case, there was recovery of matrix Gla protein RNA and osteopontin RNA expression. Still other cells were incubated in neutral differentiation medium for 1, 2, or 3 wk and then transferred to acid medium; in each case there was inhibition of matrix Gla protein RNA and osteopontin RNA expression. Thus metabolic acidosis appears to specifically inhibit RNA accumulation of certain genes whose products may be essential for formation of mature bone matrix.

calcium; bone; hydrogen ion; mineralization


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
K. K. Frick, K. LaPlante, and D. A. Bushinsky
RANK ligand and TNF-{alpha} mediate acid-induced bone calcium efflux in vitro
Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1005 - F1011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Bushinsky, S. B. Smith, K. L. Gavrilov, L. F. Gavrilov, J. Li, and R. Levi-Setti
Chronic acidosis-induced alteration in bone bicarbonate and phosphate
Am J Physiol Renal Physiol, September 1, 2003; 285(3): F532 - F539.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Bushinsky, S. B. Smith, K. L. Gavrilov, L. F. Gavrilov, J. Li, and R. Levi-Setti
Acute acidosis-induced alteration in bone bicarbonate and phosphate
Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1091 - F1097.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. A. Greenwald, B. J. Mehrara, J. A. Spector, S. M. Warren, P. J. Fagenholz, L. P. Smith, P. J. Bouletreau, F. E. Crisera, H. Ueno, and M. T. Longaker
In Vivo Modulation of FGF Biological Activity Alters Cranial Suture Fate
Am. J. Pathol., February 1, 2001; 158(2): 441 - 452.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. A. Spector, B. J. Mehrara, J. A. Greenwald, P. B. Saadeh, D. S. Steinbrech, P. J. Bouletreau, L. P. Smith, and M. T. Longaker
Osteoblast expression of vascular endothelial growth factor is modulated by the extracellular microenvironment
Am J Physiol Cell Physiol, January 1, 2001; 280(1): C72 - C80.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. S. Krieger, W. R. Parker, K. M. Alexander, and D. A. Bushinsky
Prostaglandins regulate acid-induced cell-mediated bone resorption
Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1077 - F1082.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. K. Frick and D. A. Bushinsky
In vitro metabolic and respiratory acidosis selectively inhibit osteoblastic matrix gene expression
Am J Physiol Renal Physiol, November 1, 1999; 277(5): F750 - F755.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Bushinsky, J. M. Chabala, K. L. Gavrilov, and R. Levi-Setti
Effects of in vivo metabolic acidosis on midcortical bone ion composition
Am J Physiol Renal Physiol, November 1, 1999; 277(5): F813 - F819.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Bushinsky, W. R. Parker, K. M. Alexander, and N. S. Krieger
Metabolic, but not respiratory, acidosis increases bone PGE2 levels and calcium release
Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1058 - F1066.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online