AJP - Renal Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 275: F863-F869, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, D.
Right arrow Articles by Aperia, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, D.
Right arrow Articles by Aperia, A.
Vol. 275, Issue 6, F863-F869, December 1998

Effects of okadaic acid, calyculin A, and PDBu on state of phosphorylation of rat renal Na+-K+-ATPase

Dailin Li1, Sam Xian Jun Cheng1, Gilberto Fisone2, Michael J. Caplan3, Yoshiyuki Ohtomo1, and Anita Aperia1

Departments of Woman and Child Health, 1 Pediatric Unit, and 2 Neuroscience, Karolinska Institute, S-171 76 Stockholm, Sweden; and 3 Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510

Several indirect lines of evidence suggest that protein kinases and phosphatases modulate the activity of renal Na+-K+-ATPase. The aim of this study was to examine whether such regulation may occur via modulation of the state of phosphorylation of Na+-K+-ATPase. Slices from rat renal cortex were prelabeled with [32P]orthophosphate and incubated with the inhibitors of protein phosphatase (PP)-1 and PP-2A, okadaic acid (OA) and calyculin A (CL-A), respectively, the protein kinase C (PKC) activator, phorbol 12,13-dibutyrate (PDBu), or the PP-2B inhibitor, FK-506. Phosphorylation of Na+-K+-ATPase alpha -subunit was evaluated by measuring the amount of [32P]phosphate incorporation into the immunoprecipitated protein. Incubation with either OA, CL-A, or PDBu caused four- to fivefold increases in the amount of [32P]phosphate incorporation into immunoprecipitated Na+-K+-ATPase alpha -subunit. OA and PDBu had a synergistic effect on the state of phosphorylation of Na+-K+-ATPase alpha -subunit. FK-506 did not affect Na+-K+-ATPase phosphorylation, neither alone nor in the presence of PDBu. Each of the drugs, OA, CL-A, and PDBu, inhibited the activity of Na+-K+-ATPase in microdissected proximal tubules. PDBu potentiated OA-induced inhibition of Na+-K+-ATPase activity. Inhibition of Na+-K+-ATPase required a lower dose of CL-A than of OA. On the basis of the inhibitory constant values of CL-A and OA for PP-1 and PP-2A, it is concluded that the tubular effect is mainly due to inhibition of PP-1. The PP-1 activity in rat renal cortex was ~1.5 nmol Pi · mg protein-1 · min-1. Using a monoclonal anti-alpha antibody that fails to recognize the subunit when Ser23 is phosphorylated by PKC, we demonstrated that the dose response of PDBu inhibition of Na+-K+-ATPase correlated with the dose response of phosphorylation of the enzyme. The results suggest that the state of phosphorylation and activity of proximal tubular Na+-K+-ATPase are determined by the balance between the activities of protein kinases and phosphatases.

renal cortical tissue; proximal convoluted tubule; phorbol 12,13-dibutyrate


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Mercado, N. Vazquez, L. Song, R. Cortes, A. H. Enck, R. Welch, E. Delpire, G. Gamba, and D. B. Mount
NH2-terminal heterogeneity in the KCC3 K+-Cl- cotransporter
Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1246 - F1261.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. Sweeney, W. Niu, V. A. Canfield, R. Levenson, and A. Klip
Insulin increases plasma membrane content and reduces phosphorylation of Na+-K+ pump alpha 1-subunit in HEK-293 cells
Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1797 - C1803.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. E. MAGYAR, Y. ZHANG, N.-H. HOLSTEIN-RATHLOU, and A. A. MCDONOUGH
Downstream Shift in Sodium Pump Activity along the Nephron during Acute Hypertension
J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2231 - 2240.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. Feraille and A. Doucet
Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control
Physiol Rev, January 1, 2001; 81(1): 345 - 418.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. G. Therien and R. Blostein
Mechanisms of sodium pump regulation
Am J Physiol Cell Physiol, September 1, 2000; 279(3): C541 - C566.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Glavy, S. M. Wu, P. J. Wang, G. A. Orr, and A. W. Wolkoff
Down-regulation by Extracellular ATP of Rat Hepatocyte Organic Anion Transport Is Mediated by Serine Phosphorylation of Oatp1
J. Biol. Chem., January 14, 2000; 275(2): 1479 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Mercado, L. Song, N. Vazquez, D. B. Mount, and G. Gamba
Functional Comparison of the K+-Cl- Cotransporters KCC1 and KCC4
J. Biol. Chem., September 22, 2000; 275(39): 30326 - 30334.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Feschenko, E. Stevenson, and K. J. Sweadner
Interaction of Protein Kinase C and cAMP-dependent Pathways in the Phosphorylation of the Na,K-ATPase
J. Biol. Chem., October 27, 2000; 275(44): 34693 - 34700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Gomes and P. Soares-da-Silva
Role of cAMP-PKA-PLC signaling cascade on dopamine-induced PKC-mediated inhibition of renal Na+-K+-ATPase activity
Am J Physiol Renal Physiol, June 1, 2002; 282(6): F1084 - F1096.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online