AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 275: F894-F903, 1998;
0363-6127/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Basile, D. P.
Right arrow Articles by Hammerman, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Basile, D. P.
Right arrow Articles by Hammerman, M. R.
Vol. 275, Issue 6, F894-F903, December 1998

Extracellular matrix-related genes in kidney after ischemic injury: potential role for TGF-beta in repair

David P. Basile, Daniel R. Martin, and Marc R. Hammerman

George M. O'Brien Kidney and Urological Disease Center, Renal Division, Departments of Medicine and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110

The renal expression of transforming growth factor-beta 1 (TGF-beta 1) is enhanced following induction of ischemic injury in rat. In cultured renal cells, TGF-beta stimulates the synthesis of extracellular matrix. To link TGF-beta 1 expression with the regulation of extracellular matrix postischemia, we characterized the expression of several genes known to regulate extracellular matrix synthesis at various times during recovery from acute ischemic renal injury in rat. Levels of mRNA for plasminogen activator inhibitor-1 (PAI-1), tissue inhibitor of metalloprotease-1 (TIMP-1), alpha 1(IV) collagen, and fibronectin-EIIIA (FN-EIIIA) mRNAs were significantly enhanced in kidneys within 12 h to 3 days after injury and remained elevated at 7-28 days postischemia relative to levels in kidneys of sham-operated controls. PAI-1 mRNA and peptide were localized in regenerating proximal tubules at 3 and 7 days postischemic injury. alpha 1(IV) Collagen and FN-EIIIA mRNAs were expressed primarily in regenerating proximal tubule cells. Immunoreactivity for FN-EIIIA was enhanced in the tubular basement membrane (TBM) of regenerating proximal tubules, and alpha 1(IV) collagen immunoreactivity was detected in thickened tubulointerstitial spaces. In contrast, TIMP-1 immunoreactivity was enhanced in distal nephron structures postischemia. Immunoneutralization of TGF-beta in vivo attenuated the increases in FN-EIIIA, alpha 1(IV) collagen, PAI-1, and TIMP-1 mRNAs by 52%, 73%, 43%, and 27%, respectively. These data are consistent with TGF-beta expression postischemic injury participating in renal regeneration of extracellular matrix homeostasis in the proximal TBM.

acute renal failure; differentiation; proximal tubule; regeneration; transforming growth factor-beta


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
J. M. Catania, G. Chen, and A. R. Parrish
Role of matrix metalloproteinases in renal pathophysiologies
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F905 - F911.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. G. Docherty, O. E. O'Sullivan, D. A. Healy, J. M. Fitzpatrick, and R. W. G. Watson
Evidence that inhibition of tubular cell apoptosis protects against renal damage and development of fibrosis following ureteric obstruction
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F4 - F13.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. P. Basile, K. Fredrich, M. Alausa, C. P. Vio, M. Liang, M. R. Rieder, A. S. Greene, and A. W. Cowley Jr.
Identification of persistently altered gene expression in the kidney after functional recovery from ischemic acute renal failure
Am J Physiol Renal Physiol, May 1, 2005; 288(5): F953 - F963.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. R. Spurgeon, D. L. Donohoe, and D. P. Basile
Transforming growth factor-{beta} in acute renal failure: receptor expression, effects on proliferation, cellularity, and vascularization after recovery from injury
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F568 - F577.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. P. Basile, K. Fredrich, D. Weihrauch, N. Hattan, and W. M. Chilian
Angiostatin and matrix metalloprotease expression following ischemic acute renal failure
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F893 - F902.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
P. A. Nony and R. G. Schnellmann
Mechanisms of Renal Cell Repair and Regeneration after Acute Renal Failure
J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 905 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Zuk and K. S. Matlin
Induction of a laminin isoform and alpha 3beta 1-integrin in renal ischemic injury and repair in vivo
Am J Physiol Renal Physiol, November 1, 2002; 283(5): F971 - F984.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Zuk, J. V. Bonventre, and K. S. Matlin
Expression of fibronectin splice variants in the postischemic rat kidney
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1037 - F1053.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. F. Sun, Y. Fujigaki, T. Fujimoto, K. Yonemura, and A. Hishida
Possible Involvement of Myofibroblasts in Cellular Recovery of Uranyl Acetate-Induced Acute Renal Failure in Rats
Am. J. Pathol., October 1, 2000; 157(4): 1321 - 1335.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. H. Yoo, B. A. Thornhill, and R. L. Chevalier
Angiotensin stimulates TGF-beta 1 and clusterin in the hydronephrotic neonatal rat kidney
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2000; 278(3): R640 - R645.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online