| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Woman and Child Health, Karolinska Institute, St. Göran's Children's Hospital, 112 81 Stockholm, Sweden; 2 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus, Denmark; and 3 Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York 10021-6399
Aquaporin-2 (AQP2), the protein that mediates arginine
vasopressin (AVP)-regulated apical water transport in the renal
collecting duct, possesses a single consensus phosphorylation site for
cAMP-dependent protein kinase A (PKA) at
Ser256. The aim of this study was
to examine whether AVP, and other agents that increase cAMP levels,
could stimulate the phosphorylation of AQP2 in intact rat renal tissue.
Rat renal papillae were prelabeled with
32P and incubated with vehicle or
drugs, and then AQP2 was immunoprecipitated. Two polypeptides
corresponding to nonglycosylated (29 kDa) and glycosylated (35-48
kDa) AQP2 were identified by SDS-PAGE. AVP caused a time- and
dose-dependent increase in phosphorylation of both glycosylated and
nonglycosylated AQP2. The threshold dose for a significant increase in
phosphorylation was 10 pM, which corresponds to a physiological serum
concentration of AVP. Maximal phosphorylation was reached within 1 min
of AVP incubation. This effect on AQP2 phosphorylation was mimicked by
the vasopressin (V2) agonist,
1-desamino-[8-D-arginine]vasopressin
(DDAVP), or forskolin. Two-dimensional phosphopeptide mapping indicated
that AVP and forskolin stimulated the phosphorylation of the same site in AQP2. Immunoblot analysis using a phosphorylation state-specific antiserum revealed an increase in phosphorylation of
Ser256 after incubation of
papillae with AVP. The results indicate that AVP stimulates
phosphorylation of AQP2 at Ser256
via activation of PKA, supporting the idea that this is one of the
first steps leading to increased water permeability in collecting duct cells.
adenosine 3',5'-cyclic monophosphate; collecting duct cells; protein kinase A; vasopressin receptor; water permeability
This article has been cited by other articles:
|
|
 |
|
  U. Hasler Controlled aquaporin-2 expression in the hypertonic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C641 - C653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. B. Moeller, N. MacAulay, M. A. Knepper, and R. A. Fenton Role of multiple phosphorylation sites in the COOH-terminal tail of aquaporin-2 for water transport: evidence against channel gating Am J Physiol Renal Physiol, March 1, 2009; 296(3): F649 - F657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Sachs, T. Pisitkun, J. D. Hoffert, M.-J. Yu, and M. A. Knepper LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1799 - F1806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Yu, T. Pisitkun, G. Wang, J. F. Aranda, P. A. Gonzales, D. Tchapyjnikov, R.-F. Shen, M. A. Alonso, and M. A. Knepper Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct Am J Physiol Cell Physiol, September 1, 2008; 295(3): C661 - C678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Procino, C. Barbieri, G. Tamma, L. De Benedictis, J. E. Pessin, M. Svelto, and G. Valenti AQP2 exocytosis in the renal collecting duct - involvement of SNARE isoforms and the regulatory role of Munc18b J. Cell Sci., June 15, 2008; 121(12): 2097 - 2106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mutig, A. Paliege, T. Kahl, T. Jons, W. Muller-Esterl, and S. Bachmann Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1166 - F1177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Shi, X. R. Cao, J. Qu, K. A. Volk, P. Kirby, R. A. Williamson, J. B. Stokes, and B. Yang Nephrogenic diabetes insipidus in mice caused by deleting COOH-terminal tail of aquaporin-2 Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1334 - F1344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hoffert, J. Nielsen, M.-J. Yu, T. Pisitkun, S. M. Schleicher, S. Nielsen, and M. A. Knepper Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct Am J Physiol Renal Physiol, February 1, 2007; 292(2): F691 - F700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dimke, A. Flyvbjerg, S. Bourgeois, K. Thomsen, J. Frokiaer, P. Houillier, S. Nielsen, and S. Frische Acute growth hormone administration induces antidiuretic and antinatriuretic effects and increases phosphorylation of NKCC2 Am J Physiol Renal Physiol, February 1, 2007; 292(2): F723 - F735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Nishimoto, G. Sasaki, E. Yaoita, M. Nameta, H. Li, K. Furuse, H. Fujinaka, Y. Yoshida, A. Mitsudome, and T. Yamamoto Molecular characterization of water-selective AQP (EbAQP4) in hagfish: insight into ancestral origin of AQP4 Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R644 - R651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Lee, I.-K. Song, K.-J. Jang, J. Nielsen, J. Frokiaer, S. Nielsen, and T.-H. Kwon Increased AQP2 targeting in primary cultured IMCD cells in response to angiotensin II through AT1 receptor Am J Physiol Renal Physiol, January 1, 2007; 292(1): F340 - F350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Jensen, C. Li, H. A. Praetorius, R. Norregaard, S. Frische, M. A. Knepper, S. Nielsen, and J. Frokiaer Angiotensin II mediates downregulation of aquaporin water channels and key renal sodium transporters in response to urinary tract obstruction Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1021 - F1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-P. Yip Epac-mediated Ca2+ mobilization and exocytosis in inner medullary collecting duct Am J Physiol Renal Physiol, October 1, 2006; 291(4): F882 - F890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Russo, M. McKee, and D. Brown Methyl-beta-cyclodextrin induces vasopressin-independent apical accumulation of aquaporin-2 in the isolated, perfused rat kidney Am J Physiol Renal Physiol, July 1, 2006; 291(1): F246 - F253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Procino, D. B. Caces, G. Valenti, and J. E. Pessin Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment Am J Physiol Renal Physiol, May 1, 2006; 290(5): F985 - F994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Kwon, J. Nielsen, M. A. Knepper, J. Frokiaer, and S. Nielsen Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats Am J Physiol Renal Physiol, April 1, 2005; 288(4): F673 - F684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lu, T.-X. Sun, R. Bouley, K. Blackburn, M. McLaughlin, and D. Brown Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2 Am J Physiol Renal Physiol, February 1, 2004; 286(2): F233 - F243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Brown The ins and outs of aquaporin-2 trafficking Am J Physiol Renal Physiol, May 1, 2003; 284(5): F893 - F901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shaw and D. Marples A rat kidney tubule suspension for the study of vasopressin-induced shuttling of AQP2 water channels Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1160 - F1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zelenina, S. Zelenin, A. A. Bondar, H. Brismar, and A. Aperia Water permeability of aquaporin-4 is decreased by protein kinase C and dopamine Am J Physiol Renal Physiol, August 1, 2002; 283(2): F309 - F318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kellenberger and L. Schild Epithelial Sodium Channel/Degenerin Family of Ion Channels: A Variety of Functions for a Shared Structure Physiol Rev, July 1, 2002; 82(3): 735 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Promeneur, T.-H. Kwon, J. Frokiar, M. A. Knepper, and S. Nielsen Vasopressin V2-receptor-dependent regulation of AQP2 expression in Brattleboro rats Am J Physiol Renal Physiol, August 1, 2000; 279(2): F370 - F382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, T.-H. Kwon, J. Frokiar, and M. A. Knepper Key Roles of Renal Aquaporins in Water Balance and Water-Balance Disorders Physiology, June 1, 2000; 15(3): 136 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zelenina, B. M. Christensen, J. Palmer, A. C. Nairn, S. Nielsen, and A. Aperia Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution Am J Physiol Renal Physiol, March 1, 2000; 278(3): F388 - F394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Christensen, M. Zelenina, A. Aperia, and S. Nielsen Localization and regulation of PKA-phosphorylated AQP2 in response to V2-receptor agonist/antagonist treatment Am J Physiol Renal Physiol, January 1, 2000; 278(1): F29 - F42. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
