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Am J Physiol Renal Physiol 276: F288-F294, 1999;
0363-6127/99 $5.00
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Vol. 276, Issue 2, F288-F294, February 1999

Renal sulfate secretion is carbonic anhydrase dependent in a marine teleost, Pleuronectes americanus

J. Larry Renfro1,4, Thomas H. Maren2,4, Cristina Zeien2,4, and Erik R. Swenson3,4

1 Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269; 2 Department of Pharmacology and Therapeutics, University of Florida Health Science Center, Gainesville, Florida 32610; 3 Department of Veterans Affairs Medical Center and University of Washington, Seattle, Washington 98108; and 4 Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672

Though chemical assays indicate that carbonic anhydrase (CA) activity is present in marine teleost nephrons, CA inhibitors have no effect on urine pH or bicarbonate excretion, parameters typically CA dependent in almost all vertebrate groups. Because marine teleost renal sulfate secretion is associated with bicarbonate anion exchange, we investigated the effect of CA inhibition on transepithelial sulfate transport by flounder renal tubule primary monolayer cultures (PTC) and on renal sulfate secretion (QSO4) by intact flounder. Both methazolamide and ethoxzolamide (10 µM) inhibited PTC secretory flux by ~50%; reabsorptive sulfate flux, Na-dependent glucose transport, and transepithelial electrical resistance were unaffected. A CA inhibitor restricted to the extracellular space (10 µM polyoxyethylene-aminobenzolamide, 3.7 kDa) had no effect on PTC sulfate transport. Intravenous administration of methazolamide reduced QSO4 almost 40% and had no effect on glomerular filtration rate (GFR), urine flow rate, or Pi excretion rate. Serum pH was significantly reduced 0.2 units, whereas urine pH was unchanged. Together, the in vitro and in vivo results indicate that CA facilitates renal sulfate secretion in the seawater teleost.

anion exchange; sulfate clearance; methazolamide; ethoxzolamide; winter flounder; fish renal function; acid-base balance


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