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Laboratory of Epithelial Transport, Departments of Internal Medicine and Pediatrics, University of Iowa; and Veterans Affairs Medical Center, Iowa City, Iowa 52242
The epithelial Na+ channel
(ENaC) plays an important role in regulating
Na+ balance in neonatal and adult
life. Using in situ hybridization, we localized 
-, 
-, and 
-rat
ENaC (rENaC) mRNA in developing rat kidney and
uroepithelia. rENaC mRNA was first detectable on fetal day 16, and by
fetal day 17, mRNA was abundant in the
terminal collecting duct and uroepithelia. After birth, the intensity
of the signals for all three subunits increased in the cortical
collecting ducts and by 9 days after birth had diminished in the inner
medullary collecting ducts. Expression in uroepithelial cells was
different. mRNA for - and -rENaC, but not -rENaC, was detected
in pelvis, ureters, and bladder at all stages of development beyond
fetal day 16. By RNase protection
assay (RPA), the greatest increase in subunit abundance in the kidney
occurred before birth. Between postnatal days
9 and 30, the
abundance of - and -rENaC decreased relative to -rENaC in
outer and inner medulla. The urinary bladder, in contrast, demonstrated
the greatest increase in - and -rENaC mRNA abundance
after birth. We were generally unable to detect -rENaC by RPA in
urinary bladder. Feeding weaned rats a diet of high or low NaCl did not
change the abundance of any of the subunit mRNAs in bladder. These
results demonstrate additional heterogeneity of developmental
expression and regulation of ENaC. The differences between the
collecting duct and uroepithelial cell rENaC mRNA regulation raise the
possibility of significant differences in function.
RNase protection assay; collecting duct; urinary bladder; ureter; pelvis; in situ hybridization
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