| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232; 4 Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and 3 Molecular Physiology Unit, Department of Nephrology and Mineral Metabolism, Instituto Nacional de la Nutrición Salvador Zubirán and Instituto de Investigaciones Biomédicas, National University of Mexico, Mexico City CP 14000, Mexico; 2 Department of Medical Physiology, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
We have identified several alternatively spliced cDNAs encoding
mBSC1, an apical bumetanide-sensitive
Na+-K+-2Cl
cotransporter from mouse kidney. Two full-length clones were isolated,
designated C4 and C9, predicting proteins of 770 and 1,095 amino acids,
respectively. The C4 isoforms are generated by utilization of an
alternative polyadenylation site located within the intron between
exons 16 and 17 of the mBSC1 gene on chromosome 2; the resultant
transcripts predict a truncated COOH terminus ending in a unique 55 amino acid sequence. The predicted C4 and C9 COOH termini differ in the
distribution of putative phosphorylation sites for both protein kinase
A and C. Independent splicing events involve three previously described
cassette exons, which are predicted to encode most of the second
transmembrane domain. A total of six different isoforms are expressed,
generated by the combinatorial association of three cassette exons and
two alternative 3' ends. C9-specific and C4-specific antibodies
detect proteins of ~150 and 120 kDa, respectively, in mouse kidney.
Immunofluorescence and immunohistochemistry indicate expression of both
COOH-terminal isoforms within the thick ascending limb of the loop of
Henle (TAL). However, staining with the C4 antibody is more
heterogeneous, with a decreased proportion of positive cells in the
cortical TAL. Functional expression in
Xenopus oocytes indicates a dominant negative function for C4 isoforms [companion study, C. Plata, D. B. Mount, V. Rubio, S. C. Hebert, and G. Gamba. Am. J. Physiol. 276 (Renal
Physiol. 45): F347-F358, 1999], and the
differential expression of these isoforms may contribute to functional
heterogeneity of
Na+-K+-2Cl
cotransport in mouse TAL.
sodium-potassium-chloride cotransporter; bumetanide; protein kinase A; adenosine 3',5'-cyclic monophosphate; thick ascending limb of Henle; alternative splicing
This article has been cited by other articles:
|
|
 |
|
  H. Castrop and J. Schnermann Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and functional significance Am J Physiol Renal Physiol, October 1, 2008; 295(4): F859 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Angelow, R. El-Husseini, S. A. Kanzawa, and A. S. L. Yu Renal localization and function of the tight junction protein, claudin-19 Am J Physiol Renal Physiol, July 1, 2007; 293(1): F166 - F177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mercado, N. Vazquez, L. Song, R. Cortes, A. H. Enck, R. Welch, E. Delpire, G. Gamba, and D. B. Mount NH2-terminal heterogeneity in the KCC3 K+-Cl- cotransporter Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1246 - F1261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gamba Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters Physiol Rev, April 1, 2005; 85(2): 423 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tovar-Palacio, N. A. Bobadilla, P. Cortes, C. Plata, P. de los Heros, N. Vazquez, and G. Gamba Ion and diuretic specificity of chimeric proteins between apical Na+-K+-2Cl- and Na+-Cl- cotransporters Am J Physiol Renal Physiol, September 1, 2004; 287(3): F570 - F577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Meade, R. S. Hoover, C. Plata, N. Vazquez, N. A. Bobadilla, G. Gamba, and S. C. Hebert cAMP-dependent activation of the renal-specific Na+-K+-2Cl- cotransporter is mediated by regulation of cotransporter trafficking Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1145 - F1154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Shankar and D. C. Brater Loop diuretics: from the Na-K-2Cl transporter to clinical use Am J Physiol Renal Physiol, January 1, 2003; 284(1): F11 - F21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Klein, D. Le Quach, J. M. Cole, K. Disher, A. K. Mongiu, X. Wang, K. E. Bernstein, and J. M. Sands Impaired urine concentration and absence of tissue ACE: involvement of medullary transport proteins Am J Physiol Renal Physiol, September 1, 2002; 283(3): F517 - F524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Plata, P. Meade, N. Vazquez, S. C. Hebert, and G. Gamba Functional Properties of the Apical Na+-K+-2Cl- Cotransporter Isoforms J. Biol. Chem., March 22, 2002; 277(13): 11004 - 11012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gamba Alternative splicing and diversity of renal transporters Am J Physiol Renal Physiol, November 1, 2001; 281(5): F781 - F794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Plata, P. Meade, A. Hall, R. C. Welch, N. Vazquez, S. C. Hebert, and G. Gamba Alternatively spliced isoform of apical Na+-K+-Cl{-} cotransporter gene encodes a furosemide-sensitive Na+-Cl{-}cotransporter Am J Physiol Renal Physiol, April 1, 2001; 280(4): F574 - F582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Attmane-Elakeb, H. Amlal, and M. Bichara Ammonium carriers in medullary thick ascending limb Am J Physiol Renal Physiol, January 1, 2001; 280(1): F1 - F9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Plata, D. B. Mount, V. Rubio, S. C. Hebert, and G. Gamba Isoforms of the Na-K-2Cl cotransporter in murine TAL II. Functional characterization and activation by cAMP Am J Physiol Renal Physiol, March 1, 1999; 276(3): F359 - F366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing, D. Loffing-Cueni, V. Valderrabano, L. Klausli, S. C. Hebert, B. C. Rossier, J. G. J. Hoenderop, R. J. M. Bindels, and B. Kaissling Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1021 - F1027. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
