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Am J Physiol Renal Physiol 276: F398-F408, 1999;
0363-6127/99 $5.00
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Vol. 276, Issue 3, F398-F408, March 1999

A novel p64-related Clminus channel: subcellular distribution and nephron segment-specific expression

John C. Edwards

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Several closely related proteins that have been implicated as chloride channels of intracellular membranes have recently been described. We report here the molecular cloning and characterization of a new member of this family from human cells. On the basis of sequence similarity, we conclude that this new protein represents the human version of a previously described protein from rat brain named p64H1. The human version of p64H1 (huH1) is a 28.7-kDa protein that shows an apparent molecular mass of 31 kDa by SDS-PAGE. A single 4.5-kb message is detected on Northern blots and is present in all tissues probed. The protein is expressed in an intracellular vesicular pattern in Panc-1 cells that is distinct from the endoplasmic reticulum, fluid-phase endocytic, and transferrin-recycling compartments, but which does colocalize with caveolin. In human kidney, huH1 is highly expressed in a diffuse pattern in the apical domain of proximal tubule cells. huH1 is expressed less abundantly in a vesicular pattern in glomeruli and distal nephron.

caveolin; caveolae; p64H1; chloride intracellular channel 2; NCC27; proximal tubule


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