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1 Department of Anatomy, Catholic University Medical College, Seoul, Korea; 2 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-0951; 3 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus, Denmark; and 4 Division of Nephrology, Hypertension and Transplantation, University of Florida College of Medicine, Gainesville, Florida 32610-0224
Aquaporin 1 (AQP-1) is a water channel protein that is
constitutively expressed in renal proximal tubule and descending thin limb cells as well as in endothelial cells of the descending vasa recta. Studies in the developing rat kidney have demonstrated that
AQP-1 is expressed in renal tubules before birth. However, nothing is
known about the expression of AQP-1 in the renal vasculature during
kidney development. The purpose of this study was to establish the
distribution of AQP-1 in the renal vasculature of the developing rat
kidney and follow the differentiation of the vascular system during
kidney development. Kidneys from 16-, 17-, 18-, and 20-day-old fetuses
and 1-, 4-, 7-, 14-, 21-, and 28-day-old pups were preserved and
processed for immunohistochemical studies using a preembedding immunoperoxidase procedure. AQP-1 immunoreactivity was detected using
affinity-purified rabbit polyclonal antibodies to AQP-1. AQP-1 was
expressed throughout the arterial portion of the renal vasculature of
the fetal and neonatal kidney from gestational age 17 days to 1 wk
after birth. AQP-1 immunoreactivity gradually disappeared from the
renal vasculature between 1 and 2 wk of age and remained only in the
descending vasa recta. In contrast, AQP-1 immunoreactivity was not
observed in lymphatic vessels until 3 wk of age and persisted in the
adult kidney. AQP-1 was also expressed in a population of interstitial
cells in the terminal part of the renal papilla at 3 wk of
age as well as in the adult kidney. The transient expression
of AQP-1 in the arterial portion of the renal vasculature in the
developing rat kidney suggests that AQP-1 is important for fluid
equilibrium and/or drainage in the developing kidney or,
alternatively, plays a role in the regulation of growth and/or
branching of the vascular tree during kidney development.
kidney development; vasa recta; renal lymphatics; immunohistochemistry
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