AJP - Renal AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 276: F535-F543, 1999;
0363-6127/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grüning, W.
Right arrow Articles by Walz, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grüning, W.
Right arrow Articles by Walz, G.
Vol. 276, Issue 4, F535-F543, April 1999

Modulation of renal tubular cell function by RGS3

W. Grüning1, T. Arnould1, F. Jochimsen1, L. Sellin1, S. Ananth1, E. Kim2, and G. Walz1

1 Renal Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston 02215; and 2 Laboratory of Molecular and Developmental Neuroscience, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114

The recently discovered family of regulators of G protein signaling (RGS) accelerates the intrinsic GTPase activity of certain Galpha subunits, thereby terminating G protein signaling. Particularly high mRNA levels of one family member, RGS3, are found in the adult kidney. To establish the temporal and spatial renal expression pattern of RGS3, a polyclonal antiserum was raised against the COOH terminus of RGS3. Staining of mouse renal tissue at different gestational stages revealed high levels of RGS3 within the developing and mature tubular epithelial cells. We tested whether RGS3 can modulate tubular migration, an important aspect of tubular development, in response to G protein-mediated signaling. Several mouse intermedullary collecting duct (mIMCD-3) cell lines were generated that expressed RGS3 under the control of an inducible promoter. Lysophosphatidic acid (LPA) is a potent chemoattractant that mediates its effects through heterotrimeric G proteins. We found that induction of RGS3 significantly reduced LPA-mediated cell migration in RGS3-expressing mIMCD-3 clones, whereas chemotaxis induced by hepatocyte growth factor remained unaffected by RGS3. Our findings suggest that RGS3 modulates tubular functions during renal development and in the adult kidney.

renal tubular epithelial cells; kidney development; chemotaxis; G protein; regulators of G protein signaling


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Huang, A. Sindic, C. E. Hill, K. M. Hujer, K. W. Chan, M. Sassen, Z. Wu, Y. Kurachi, S. Nielsen, M. F. Romero, et al.
Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F1073 - F1081.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
S. Hollinger and J. R. Hepler
Cellular Regulation of RGS Proteins: Modulators and Integrators of G Protein Signaling
Pharmacol. Rev., September 1, 2002; 54(3): 527 - 559.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H. Zhong and R. R. Neubig
Regulator of G Protein Signaling Proteins: Novel Multifunctional Drug Targets
J. Pharmacol. Exp. Ther., June 1, 2001; 297(3): 837 - 845.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
K. Reif and J. G. Cyster
RGS Molecule Expression in Murine B Lymphocytes and Ability to Down-Regulate Chemotaxis to Lymphoid Chemokines ,2
J. Immunol., May 1, 2000; 164(9): 4720 - 4729.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. O. Dulin, P. Pratt, C. Tiruppathi, J. Niu, T. Voyno-Yasenetskaya, and M. J. Dunn
Regulator of G Protein Signaling RGS3T Is Localized to the Nucleus and Induces Apoptosis
J. Biol. Chem., July 7, 2000; 275(28): 21317 - 21323.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online