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1 Second Department of Internal
Medicine,
To gain insight into the physiological role of a
kidney-specific chloride channel, CLC-K2, the exact
intrarenal localization was determined by in situ hybridization. In
contrast to the inner medullary localization of CLC-K1, the signal of
CLC-K2 in our in situ hybridization study was highly evident in the
superficial cortex, moderate in the outer medulla, and absent in the
inner medulla. To identify the nephron segments where CLC-K2 mRNA was expressed, we performed in situ hybridization of CLC-K2 and
immunohistochemistry of marker proteins
(Na+/Ca2+
exchanger,
Na+-Cl
cotransporter, aquaporin-2 water channel, and Tamm-Horsfall
glycoprotein) in sequential sections of a rat kidney. Among the tubules
of the superficial cortex, CLC-K2 mRNA was highly expressed in the
distal convoluted tubules, connecting tubules, and cortical collecting ducts. The expression of CLC-K2 in the outer and inner medullary collecting ducts was almost absent. In contrast, a moderate signal of
CLC-K2 mRNA was observed in the medullary thick ascending limb of
Henle's loop, but the signal in the cortical thick ascending limb of
Henle's loop was low. These results clearly demonstrated that CLC-K2
was not colocalized with CLC-K1 and that its localization along the
nephron segments was relatively broad compared with that of CLC-K1.
in situ hybridization; sodium/calcium exchanger; aquaporin-2 water channel; sodium-chloride cotransporter; Tamm-Horsfall glycoprotein
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