To functionally characterize transport properties of the apical anion exchanger of rabbit -intercalated cells, the mean change in anion exchange activity, dpH_i/dt (where pH_i is intracellular pH), was measured in response to lumen Cl replacement with gluconate in perfused cortical collecting ducts (CCDs). -Cell apical anion exchange was not affected by 15-min exposure to 0.2 mM lumen DIDS in the presence of 115 mM Cl. In contrast, apical anion exchange was significantly inhibited by 0.1 mM lumen DIDS in the absence of Cl. -Cell apical anion exchange was unchanged by 15 mM maleic anhydride, 10 mM phenylglyoxal, 0.2 mM niflumic acid, 1 mM edecrin, 1 mM furosemide, 1 mM probenecid, or 0.1 mM diphenylamine-2-carboxylate. However, -cell apical anion exchange was inhibited by -cyano-4-hydroxycinnamic acid, with an IC₅₀ of 2.4 mM. Substitution of either sulfate or gluconate for lumen Cl resulted in a similar rate of alkalinization. Conversely, pH_i was unchanged by substitution of sulfate for lumen gluconate, confirming the lack of transport of sulfate on the -cell apical anion exchanger. Taken together, the results demonstrate a distinct "fingerprint" of the rabbit CCD -cell apical anion exchanger that is unlike that of other known anion exchangers.

intercalated cell; anion exchange; intracellular pH; acid-base transport

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