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2-subunit and apical localization of
Na+-K+-ATPase
in metanephric kidney
1 Division of Nephrology, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029; and 2 Division of Nephrology, St. Luc Academic Hospital, University of Louvain Medical School, B-1200 Brussels, Belgium
During kidney organogenesis, the
Na+-K+-ATPase
pump is not restricted to the basolateral plasma membrane of the renal
epithelial cell but is instead either localized to the apical and
lateral membrane sites of the early nephron or expressed in a
nonpolarized distribution in the newly formed collecting ducts. The
importance of
Na+-K+-ATPase
-subunit expression in the translocation of the
Na+-K+-ATPase
to the plasma membrane raises the question as to which -subunit
isoform is expressed during kidney organogenesis. Immunocytochemical, Western analysis and RNase protection studies showed that both 2-subunit protein and 2 mRNA are expressed in the early gestation to midgestation human metanephric kidney. In contrast, although 1
mRNA abundance is equivalent to that of the 2-subunit in the metanephric kidney, the 1-subunit protein was not detected in early
to midgestation metanephric kidney samples. Immunocytochemical analysis
revealed that both 
1- and 2-subunits were present in the apical
epithelial plasma membranes of distal nephron segments of early stage
nephrons, maturing loops of Henle, and collecting ducts during kidney
development. We also detected a significant increase in 1 and 1
mRNA after birth with a marked reduction in 2 mRNA abundance
associated with an increase in 1- and 1-subunit proteins and loss
of 2 protein expression. These studies support the conclusion that
the expression of the 2-subunit in the fetal kidney may be an
important mechanism controlling polarization of the
Na+-K+-ATPase
pump in the epithelia of the developing nephron during kidney organogenesis.
sodium-potassium adenosinetriphosphatase; renal epithelium; sodium transport; nephron
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