| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Nephrology Research and Training Center, Division of Nephrology, Departments of Medicine and Physiology, University of Alabama at Birmingham, Birmingham, Alabama 35294
Previous
micropuncture studies suggested that macula densa (MD) cells might
detect variations in luminal sodium chloride concentration ([NaCl]l) through
changes in cytosolic calcium
([Ca2+]c).
To test this hypothesis, MD
[Ca2+]c
was measured with fluorescence microscopy using fura 2 in the isolated
perfused thick ascending limb with attached glomerulus preparation
dissected from rabbit kidney. Tubules were bathed and perfused with a
Ringer solution,
[NaCl]l was varied and
isosmotically replaced with
N-methyl-D-glucamine
cyclamate. Control
[Ca2+]c,
during perfusion with 25 mM NaCl and 150 mM NaCl in the bath, averaged
101.6 ± 8.2 nM (n = 21).
Increasing [NaCl]l to
150 mM elevated
[Ca2+]c
by 39.1 ± 5.2 nM (n = 21, P < 0.01). This effect was
concentration dependent between zero and 60 mM
[NaCl]l. The presence
of either luminal furosemide or basolateral nifedipine or
5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), a potent
Cl
channel blocker,
significantly reduced resting
[Ca2+]c
and abolished the increase in
[Ca2+]c
in response to increased
[NaCl]l. Nifedipine
failed to produce a similar inhibitory effect when added exclusively to
the luminal perfusate. Also, 100 nM BAY K 8644, a voltage-gated
Ca2+ channel agonist, added to the
bathing solution increased
[Ca2+]c
by 33.2 ± 8.1 nM (n = 5, P < 0.05). These
observations suggest that MD cells may detect variations in
[NaCl]l through a
signaling pathway that includes
Na+-2Cl-K+
cotransport, basolateral membrane depolarization via
Cl channels, and
Ca2+ entry through voltage-gated
Ca2+ channels.
isolated perfused tubule; fluorescence microscopy; cytosolic calcium; furosemide; voltage-gated calcium channels; tubuloglomerular feedback
This article has been cited by other articles:
|
|
 |
|
  O. Kwon, S.-M. Hong, and G. Ramesh Diminished NO generation by injured endothelium and loss of macula densa nNOS may contribute to sustained acute kidney injury after ischemia-reperfusion Am J Physiol Renal Physiol, January 1, 2009; 296(1): F25 - F33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Liu, J. L. Garvin, Y. Ren, P. J. Pagano, and O. A. Carretero Depolarization of the macula densa induces superoxide production via NAD(P)H oxidase Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1867 - F1872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Peti-Peterdi Calcium wave of tubuloglomerular feedback Am J Physiol Renal Physiol, August 1, 2006; 291(2): F473 - F480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Peti-Peterdi Multiphoton imaging of renal tissues in vitro Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1079 - F1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Komlosi, A. Fintha, and P. D. Bell Renal Cell-to-Cell Communication via Extracellular ATP Physiology, April 1, 2005; 20(2): 86 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Liu and A. E. G Persson Simultaneous changes of cell volume and cytosolic calcium concentration in macula densa cells caused by alterations of luminal NaCl concentration J. Physiol., March 15, 2005; 563(3): 895 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fraser, P. Mount, R. Hill, V. Levidiotis, F. Katsis, D. Stapleton, B. E. Kemp, and D. A. Power Regulation of the energy sensor AMP-activated protein kinase in the kidney by dietary salt intake and osmolality Am J Physiol Renal Physiol, March 1, 2005; 288(3): F578 - F586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Peti-Peterdi, A. Fintha, A. L. Fuson, A. Tousson, and R. H. Chow Real-time imaging of renin release in vitro Am J Physiol Renal Physiol, August 1, 2004; 287(2): F329 - F335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Lapointe, P. D. Bell, R. Z. Sabirov, and Y. Okada Calcium-activated nonselective cationic channel in macula densa cells Am J Physiol Renal Physiol, August 1, 2003; 285(2): F275 - F280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Peti-Peterdi, S. Morishima, P. D. Bell, and Y. Okada Two-photon excitation fluorescence imaging of the living juxtaglomerular apparatus Am J Physiol Renal Physiol, July 1, 2002; 283(1): F197 - F201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ren, H. Yu, H. Wang, O. A. Carretero, and J. L. Garvin Nystatin and valinomycin induce tubuloglomerular feedback Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1102 - F1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yang, J. M. Park, L. Arend, Y. Huang, R. Topaloglu, A. Pasumarthy, H. Praetorius, K. Spring, J. P. Briggs, and J. Schnermann Low Chloride Stimulation of Prostaglandin E2 Release and Cyclooxygenase-2 Expression in a Mouse Macula Densa Cell Line J. Biol. Chem., November 22, 2000; 275(48): 37922 - 37929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kovacs, J. Peti-Peterdi, L. Rosivall, and P. D. Bell Angiotensin II directly stimulates macula densa Na-2Cl-K cotransport via apical AT1 receptors Am J Physiol Renal Physiol, February 1, 2002; 282(2): F301 - F306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Peti-Peterdi, Z. Bebok, J.-Y. Lapointe, and P. D. Bell Novel regulation of cell [Na+] in macula densa cells: apical Na+ recycling by H-K-ATPase Am J Physiol Renal Physiol, February 1, 2002; 282(2): F324 - F329. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
