| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Vascular Biology Center, Departments of Surgery, Physiology and Endocrinology, and Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30912-2500; and 2 Pharmaceutical Discovery, Abbott Laboratories, Abbott Park, Illinois 60064-3500
Experiments were designed to elucidate
the role of endothelin B receptors (ETB) on arterial
pressure and renal function in deoxycorticosterone acetate (DOCA)-salt
hypertensive rats. Male Sprague-Dawley rats underwent uninephrectomy
and were treated with either DOCA and salt (0.9% NaCl to drink) or
placebo. DOCA-salt rats given the ETB-selective antagonist,
A-192621, for 1 wk (10 mg · kg
1 · day1
in the food) had significantly greater systolic arterial pressure compared with untreated DOCA-salt rats (208 ± 7 vs. 182 ± 4 mmHg) whereas pressure in placebo rats was unchanged. In DOCA-salt, but not
placebo rats, A-192621 significantly decreased sodium and water
excretion along with parallel decreases in food and water intake. To
determine whether the response in DOCA-salt rats was due to increased
expression of ETB receptors, endothelin receptor binding
was performed by using membranes from renal medulla. Maximum binding
(Bmax) of [125I]ET-1,
[125I]ET-3, and
[125I]IRL-1620 increased from 227 ± 42, 146 ± 28, and 21 ± 1 fmol/mg protein, respectively, in
placebo rats to 335 ± 27, 300 ± 38, and 61 ± 6 fmol/mg protein,
respectively, in DOCA-salt hypertensive rats. The fraction of receptors
that are the ETB subtype was significantly increased in
DOCA-salt (0.88 ± 0.07) compared with placebo (0.64 ± 0.01). The
difference between [125I]ET-3 and
[125I]IRL-1620 binding is consistent with
possible ETB receptor subtypes in the kidney. These results
indicate that ETB receptors in the renal medulla are
up-regulated in the DOCA-salt hypertensive rat and may serve to
maintain a lower arterial pressure by promoting salt and water excretion.
endothelin B receptors; deoxycorticosterone; hypertension; sodium diet
This article has been cited by other articles:
|
|
 |
|
  M. Li, X. Dai, S. Watts, D. Kreulen, and G. Fink Increased superoxide levels in ganglia and sympathoexcitation are involved in sarafotoxin 6c-induced hypertension Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1546 - R1554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Schneider, E. W. Inscho, and D. M. Pollock Attenuated vasoconstrictor responses to endothelin in afferent arterioles during a high-salt diet Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1208 - F1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. C. Kopp, M. Z. Cicha, and L. A. Smith Differential effects of endothelin on activation of renal mechanosensory nerves: stimulatory in high-sodium diet and inhibitory in low-sodium diet Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1545 - R1556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Khalil Dietary salt and hypertension: new molecular targets add more spice Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R509 - R513. [Full Text] [PDF]
|
|
|
|
 |
|
 G. D'Angelo, J. S. Pollock, and D. M. Pollock In vivo evidence for endothelin-1-mediated attenuation of {alpha}1-adrenergic stimulation Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1251 - H1258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zeng, U. Hopfer, L. D. Asico, G. M. Eisner, R. A. Felder, and P. A. Jose Altered AT1 Receptor Regulation of ETB Receptors in Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats Hypertension, October 1, 2005; 46(4): 926 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Garvin and M. Herrera Physiological actions of renal collecting duct endothelin Am J Physiol Renal Physiol, May 1, 2005; 288(5): F910 - F911. [Full Text] [PDF]
|
|
|
|
|
|
D. M. Pollock, J. M. Jenkins, A. K. Cook, J. D. Imig, and E. W. Inscho L-type calcium channels in the renal microcirculatory response to endothelin Am J Physiol Renal Physiol, April 1, 2005; 288(4): F771 - F777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Pollock Endothelin, Angiotensin, and Oxidative Stress in Hypertension Hypertension, April 1, 2005; 45(4): 477 - 480. [Full Text] [PDF]
|
|
|
|
|
|
A. Just, A. J. M. Olson, and W. J. Arendshorst Dual constrictor and dilator actions of ETB receptors in the rat renal microcirculation: interactions with ETA receptors Am J Physiol Renal Physiol, April 1, 2004; 286(4): F660 - F668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Vassileva, C. Mountain, and D. M. Pollock Functional Role of ETB Receptors in the Renal Medulla Hypertension, June 1, 2003; 41(6): 1359 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Taylor, C. E. Gariepy, D. M. Pollock, and J. S. Pollock Unique endothelin receptor binding in kidneys of ETB receptor deficient rats Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R674 - R681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Reinhart, L. C. Preusser, S. E. Burke, J. L. Wessale, C. D. Wegner, T. J. Opgenorth, and B. F. Cox Hypertension induced by blockade of ETB receptors in conscious nonhuman primates: role of ETA receptors Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1555 - H1561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Giulumian, M. M. Molero, V. B. Reddy, J. S. Pollock, D. M. Pollock, and L. C. Fuchs Role of ET-1 receptor binding and [Ca2+]i in contraction of coronary arteries from DOCA-salt hypertensive rats Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1944 - H1949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Bianciotti and A. J. de Bold Natriuretic peptide gene expression in DOCA-salt hypertension after blockade of type B endothelin receptor Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H1127 - H1134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Ballew and G. D. Fink Role of endothelin ETB receptor activation in angiotensin II-induced hypertension: effects of salt intake Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2218 - H2225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Ortiz and J. L. Garvin Intrarenal Transport and Vasoactive Substances in Hypertension Hypertension, September 1, 2001; 38(3): 621 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Pollock and J. S. Pollock Evidence for endothelin involvement in the response to high salt Am J Physiol Renal Physiol, July 1, 2001; 281(1): F144 - F150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. G. Katakam, J. S. Pollock, D. M. Pollock, M. R. Ujhelyi, and A. W. Miller Enhanced endothelin-1 response and receptor expression in small mesenteric arteries of insulin-resistant rats Am J Physiol Heart Circ Physiol, February 1, 2001; 280(2): H522 - H527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rothermund, S. Luckert, P. Ko{beta}mehl, M. Paul, and R. Kreutz Renal Endothelin ETA/ETB Receptor Imbalance Differentiates Salt-Sensitive From Salt-Resistant Spontaneous Hypertension Hypertension, February 1, 2001; 37(2): 275 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Giardina, G. M. Green, A. N. Rinewalt, J. P. Granger, and R. A. Khalil Role of Endothelin B Receptors in Enhancing Endothelium-Dependent Nitric Oxide-Mediated Vascular Relaxation During High Salt Diet Hypertension, February 1, 2001; 37(2): 516 - 523. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
