| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Woman and Child Health, Karolinska Institutet, Astrid Lindgren Children's Hospital, S-171 76 Stockholm, Sweden; 2 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus, Denmark; 3 Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021-6399; and 4 Laboratory of Physiological Genetics, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Lavrentyeva, 630090 Novosibirsk, Russia
Prostaglandin E2 (PGE2)
antagonizes the action of arginine vasopressin (AVP) on collecting duct
water permeability. To investigate the mechanism of this
antagonism, rat renal inner medulla (IM) was incubated with the two
hormones, and the phosphorylation and subcellular distribution of the
water channel, aquaporin-2 (AQP2) were studied. Using a phosphorylation
state-specific AQP2 antibody, we demonstrated that AVP stimulates AQP2
phosphorylation at the Ser256 protein kinase A consensus
site in a time- and dose-dependent manner. In parallel studies using a
differential centrifugation technique, we demonstrated that AVP induced
translocation of AQP2 from an intracellular vesicle-enriched fraction
to a plasma membrane-enriched fraction. PGE2
(10
7 M) added after AVP
(108 M) did not decrease AQP2
phosphorylation but reversed AVP-induced translocation of AQP2 to the
plasma membrane. Preincubation of IM with PGE2 did not
prevent the effects of AVP on AQP2 phosphorylation and trafficking.
PGE2 alone did not influence AQP2 phosphorylation and
subcellular distribution. Our data indicate that 1) recruitment of AQP2 to the plasma membrane and its retrieval to a pool of intracellular vesicles may be regulated independently, 2)
PGE2 may counteract AVP action by activation of AQP2
retrieval, 3) dephosphorylation of AQP2 is not a prerequisite
for its internalization.
inner medulla; vesicular traffic; hormonal regulation; exocytosis; endocytosis
This article has been cited by other articles:
|
|
 |
|
  S. Soodvilai, Z. Jia, M.-H. Wang, Z. Dong, and T. Yang mPGES-1 deletion impairs diuretic response to acute water loading Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1129 - F1135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Nunes, U. Hasler, M. McKee, H. A. J. Lu, R. Bouley, and D. Brown A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2 Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1476 - C1487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, J. M. Sands, D. E. Kohan, R. D. Nelson, C. F. Martin, N. G. Carlson, C. D. Kamerath, Y. Ge, J. D. Klein, and B. K. Kishore Potential role of purinergic signaling in urinary concentration in inner medulla: insights from P2Y2 receptor gene knockout mice Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1715 - F1724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ge, K. A. Strait, P. K. Stricklett, T. Yang, and D. E. Kohan Role of prostaglandins in collecting duct-derived endothelin-1 regulation of blood pressure and water excretion Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1805 - F1810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jin, Z. Wang, Y. Zhang, B. Yang, and W.-H. Wang PGE2 inhibits apical K channels in the CCD through activation of the MAPK pathway Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1299 - F1307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bachteeva, E. Fock, E. Lavrova, S. Nikolaeva, S. Gambaryan, and R. Parnova Prostaglandin E2 inhibits vasotocin-induced osmotic water permeability in the frog urinary bladder by EP1-receptor-mediated activation of NO/cGMP pathway Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R528 - R537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, S. O. Topcu, M. Diget, H. Schweer, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 activity transiently contributes to increased water and NaCl excretion in the polyuric phase after release of ureteral obstruction Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1322 - F1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. J. Kennedy, H. Xiong, S. Rahal, J. Vanderluit, R. S. Slack, Y. Zhang, Y. Guan, M. D. Breyer, and R. L. Hebert Urine concentrating defect in prostaglandin EP1-deficient mice Am J Physiol Renal Physiol, February 1, 2007; 292(2): F868 - F875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hoffert, J. Nielsen, M.-J. Yu, T. Pisitkun, S. M. Schleicher, S. Nielsen, and M. A. Knepper Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct Am J Physiol Renal Physiol, February 1, 2007; 292(2): F691 - F700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dimke, A. Flyvbjerg, S. Bourgeois, K. Thomsen, J. Frokiaer, P. Houillier, S. Nielsen, and S. Frische Acute growth hormone administration induces antidiuretic and antinatriuretic effects and increases phosphorylation of NKCC2 Am J Physiol Renal Physiol, February 1, 2007; 292(2): F723 - F735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bahr, N. Franzen, W. Oelkers, A. F H Pfeiffer, and S. Diederich Effect of exogenous glucocorticoid on osmotically stimulated antidiuretic hormone secretion and on water reabsorption in man Eur. J. Endocrinol., December 1, 2006; 155(6): 845 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Klein, O. Frohlich, M. A. Blount, C. F. Martin, T. D. Smith, and J. M. Sands Vasopressin Increases Plasma Membrane Accumulation of Urea Transporter UT-A1 in Rat Inner Medullary Collecting Ducts J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2680 - 2686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nielsen, T.-H. Kwon, J. Praetorius, J. Frokiaer, M. A. Knepper, and S. Nielsen Aldosterone increases urine production and decreases apical AQP2 expression in rats with diabetes insipidus Am J Physiol Renal Physiol, February 1, 2006; 290(2): F438 - F449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, S. Nielsen, E. Feraille, and P.-Y. Martin Posttranscriptional control of aquaporin-2 abundance by vasopressin in renal collecting duct principal cells Am J Physiol Renal Physiol, January 1, 2006; 290(1): F177 - F187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sun, N. G. Carlson, A. C. Hemmert, and B. K. Kishore P2Y2 receptor-mediated release of prostaglandin E2 by IMCD is altered in hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function Am J Physiol Renal Physiol, September 1, 2005; 289(3): F585 - F592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 inhibition prevents downregulation of key renal water and sodium transport proteins in response to bilateral ureteral obstruction Am J Physiol Renal Physiol, August 1, 2005; 289(2): F322 - F333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Kishore, C. M. Krane, R. L. Miller, H. Shi, P. Zhang, A. Hemmert, R. Sun, and R. D. Nelson P2Y2 receptor mRNA and protein expression is altered in inner medullas of hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1164 - F1172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Nejsum, M. Zelenina, A. Aperia, J. Frokiaer, and S. Nielsen Bidirectional regulation of AQP2 trafficking and recycling: involvement of AQP2-S256 phosphorylation Am J Physiol Renal Physiol, May 1, 2005; 288(5): F930 - F938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kotnik, J. Nielsen, T.-H. Kwon, C. Krzisnik, J. Frokiaer, and S. Nielsen Altered expression of COX-1, COX-2, and mPGES in rats with nephrogenic and central diabetes insipidus Am J Physiol Renal Physiol, May 1, 2005; 288(5): F1053 - F1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Murer, F. Addabbo, M. Carmosino, G. Procino, G. Tamma, G. Montini, W. Rigamonti, P. Zucchetta, M. Della Vella, A. Venturini, et al. Selective Decrease in Urinary Aquaporin 2 and Increase in Prostaglandin E2 Excretion Is Associated with Postobstructive Polyuria in Human Congenital Hydronephrosis J. Am. Soc. Nephrol., October 1, 2004; 15(10): 2705 - 2712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hadrup, J. S. Petersen, J. Praetorius, E. Meier, M. Graebe, L. Brond, D. Staahltoft, S. Nielsen, S. Christensen, D. R. Kapusta, et al. Opioid receptor-like 1 stimulation in the collecting duct induces aquaresis through vasopressin-independent aquaporin-2 downregulation Am J Physiol Renal Physiol, July 1, 2004; 287(1): F160 - F168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lu, T.-X. Sun, R. Bouley, K. Blackburn, M. McLaughlin, and D. Brown Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2 Am J Physiol Renal Physiol, February 1, 2004; 286(2): F233 - F243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Ivarsen, J Frokiaer, N K Aagaard, E F Hansen, F Bendtsen, S Nielsen, and H Vilstrup Increased urinary excretion of aquaporin 2 in patients with liver cirrhosis Gut, August 1, 2003; 52(8): 1194 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Brown The ins and outs of aquaporin-2 trafficking Am J Physiol Renal Physiol, May 1, 2003; 284(5): F893 - F901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. T. Deen, B. W. M. Van Balkom, P. J. M. Savelkoul, E.-J. Kamsteeg, M. Van Raak, M. L. Jennings, T. R. Muth, V. Rajendran, and M. J. Caplan Aquaporin-2: COOH terminus is necessary but not sufficient for routing to the apical membrane Am J Physiol Renal Physiol, February 1, 2002; 282(2): F330 - F340. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
