AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 278: F417-F424, 2000;
0363-6127/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sheikh-Hamad, D.
Right arrow Articles by Yang, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sheikh-Hamad, D.
Right arrow Articles by Yang, Y.
Vol. 278, Issue 3, F417-F424, March 2000

Regulation of stanniocalcin in MDCK cells by hypertonicity and extracellular calcium

David Sheikh-Hamad, Diane Rouse, and Yu Yang

Renal Section, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030

Differential display RT-PCR cloning method was applied to poly(A)+ RNA isolated from Madin-Darby canine kidney (MDCK) cells in isotonic or hypertonic medium. A differentially expressed 360-bp PCR fragment was isolated, subcloned, sequenced, and used to screen an MDCK cDNA library constructed in lambda ZapII. A composite sequence of two overlapping cDNA clones provided 1,053 bp of sequence that was 93% identical to human stanniocalcin and corresponded to the 3'-end of the mRNA. Although the fish homolog of this hormone inhibits calcium uptake by the gill and intestine, the function of mammalian stanniocalcin remains unknown. Stanniocalcin cDNA probe hybridizes to a 4.4-kb mRNA that is induced eightfold by hypertonicity, in a manner that is dependent on medium organic osmolytes. The mRNA induction correlates with increased total cellular content of the protein and its concomitant release to the medium, consistent with secretion for autocrine or paracrine activity. Furthermore, induction of the mRNA by hypertonicity is dependent on extracellular calcium and displays a threshold phenomenon. The data suggest that kidney stanniocalcin may have a role in the adaptation of kidney cells to osmotic stress, in a manner that is extracellular calcium dependent.

kidney; osmotic stress; calcium; Madin-Darby canine kidney cells; thick ascending limb


This article has been cited by other articles:


Home page
 J EndocrinolHome page
S. Raulic, Y. Ramos-Valdes, and G. E DiMattia
Stanniocalcin 2 expression is regulated by hormone signalling and negatively affects breast cancer cell viability in vitro
J. Endocrinol., June 1, 2008; 197(3): 517 - 529.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. Sazonova, K. A. James, C. R. McCudden, D. Segal, A. Talebian, and G. F. Wagner
Stanniocalcin-1 secretion and receptor regulation in kidney cells
Am J Physiol Renal Physiol, April 1, 2008; 294(4): F788 - F794.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. B. Burg, J. D. Ferraris, and N. I. Dmitrieva
Cellular Response to Hyperosmotic Stresses
Physiol Rev, October 1, 2007; 87(4): 1441 - 1474.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Ito, J. R. Walker, C. S. Thompson, I. Moroz, W. Lin, M. L. Veselits, A. M. Hakim, A. A. Fienberg, and G. Thinakaran
Characterization of Stanniocalcin 2, a Novel Target of the Mammalian Unfolded Protein Response with Cytoprotective Properties
Mol. Cell. Biol., November 1, 2004; 24(21): 9456 - 9469.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Kanellis, R. Bick, G. Garcia, L. Truong, C. C. Tsao, D. Etemadmoghadam, B. Poindexter, L. Feng, R. J. Johnson, and D. Sheikh-Hamad
Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis
Am J Physiol Renal Physiol, February 1, 2004; 286(2): F356 - F362.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Sheikh-Hamad, R. Bick, G.-Y. Wu, B. M. Christensen, P. Razeghi, B. Poindexter, H. Taegtmeyer, A. Wamsley, R. Padda, M. Entman, et al.
Stanniocalcin-1 is a naturally occurring L-channel inhibitor in cardiomyocytes: relevance to human heart failure
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H442 - H448.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Ishibashi and M. Imai
Prospect of a stanniocalcin endocrine/paracrine system in mammals
Am J Physiol Renal Physiol, March 1, 2002; 282(3): F367 - F375.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online