| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
Aldosterone is the major corticosteroid
regulating Na+ absorption in tight epithelia and acts
primarily by activating the epithelial Na+ channel (ENaC)
through unknown induced proteins. Recently, it has been reported that
aldosterone induces the serum- and glucocorticoid-dependent kinase
sgk and that coexpressing ENaC with this kinase in Xenopus laevis oocytes increases the amiloride-sensitive Na+
current (Chen SY, Bhargava A, Mastroberardino L, Meijer OC, Wang J,
Buse P, Firestone GL, Verrey F, and Pearce D. Proc Natl Acad Sci
USA 96: 2514-2519, 1999). The present study was done to
further characterize regulation of sgk by aldosterone in native
mammalian epithelia and to examine its effect on ENaC. With both in
vivo and in vitro protocols, an almost fivefold increase in the
abundance of sgk mRNA has been demonstrated in rat kidney and
colon but not in lung. Induction of sgk by aldosterone was
detected in kidney cortex and medulla, whereas the papilla expressed a
constitutively high level of the kinase. The increase in sgk
mRNA was detected as early as 30 min after the hormonal application and
was independent of de novo protein synthesis. The observed aldosterone
dose-response relationships suggest that the response is mediated, at
least in part, by occupancy of the mineralocorticoid receptor.
Coexpressing sgk and ENaC in Xenopus oocytes evoked a
fourfold increase in the amiloride-blockable Na+ channel
activity. A point mutation in the 
-subunit known to impair
regulation of the channel by Nedd4 (Y618A) had no significant effect on
the response to sgk.
epithelial sodium channel; Xenopus laevis oocytes; kidney collecting duct
This article has been cited by other articles:
|
|
 |
|
  T. F. Ackermann, K. M. Boini, H. Volkl, M. Bhandaru, P. M. Bareiss, L. Just, V. Vallon, K. Amann, D. Kuhl, Y. Feng, et al. SGK1-sensitive renal tubular glucose reabsorption in diabetes Am J Physiol Renal Physiol, April 1, 2009; 296(4): F859 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Artunc, O. Nasir, K. Amann, K. M. Boini, H.-U. Haring, T. Risler, and F. Lang Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1624 - F1634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bhalla and K. R. Hallows Mechanisms of ENaC Regulation and Clinical Implications J. Am. Soc. Nephrol., October 1, 2008; 19(10): 1845 - 1854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Naray-Fejes-Toth, C. Boyd, and G. Fejes-Toth Regulation of epithelial sodium transport by promyelocytic leukemia zinc finger protein Am J Physiol Renal Physiol, July 1, 2008; 295(1): F18 - F26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Fejes-Toth, G. Frindt, A. Naray-Fejes-Toth, and L. G. Palmer Epithelial Na+ channel activation and processing in mice lacking SGK1 Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1298 - F1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Huang, K. M. Boini, H. Osswald, B. Friedrich, F. Artunc, S. Ullrich, J. Rajamanickam, M. Palmada, P. Wulff, D. Kuhl, et al. Resistance of mice lacking the serum- and glucocorticoid-inducible kinase SGK1 against salt-sensitive hypertension induced by a high-fat diet Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1264 - F1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lang, C. Bohmer, M. Palmada, G. Seebohm, N. Strutz-Seebohm, and V. Vallon (Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms. Physiol Rev, October 1, 2006; 86(4): 1151 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Boini, A. M. Hennige, D. Y. Huang, B. Friedrich, M. Palmada, C. Boehmer, F. Grahammer, F. Artunc, S. Ullrich, D. Avram, et al. Serum- and glucocorticoid-inducible kinase 1 mediates salt sensitivity of glucose tolerance. Diabetes, July 1, 2006; 55(7): 2059 - 2066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Grahammer, G. Henke, C. Sandu, R. Rexhepaj, A. Hussain, B. Friedrich, T. Risler, M. Metzger, L. Just, T. Skutella, et al. Intestinal function of gene-targeted mice lacking serum- and glucocorticoid-inducible kinase 1 Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1114 - G1123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Staub and D. Rotin Role of Ubiquitylation in Cellular Membrane Transport Physiol Rev, April 1, 2006; 86(2): 669 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Mullins, M. A. Bailey, and J. J. Mullins Hypertension, Kidney, and Transgenics: A Fresh Perspective Physiol Rev, April 1, 2006; 86(2): 709 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Grahammer, F. Artunc, D. Sandulache, R. Rexhepaj, B. Friedrich, T. Risler, J. A. McCormick, K. Dawson, J. Wang, D. Pearce, et al. Renal function of gene-targeted mice lacking both SGK1 and SGK3 Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R945 - R950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Huang, K. M. Boini, B. Friedrich, M. Metzger, L. Just, H. Osswald, P. Wulff, D. Kuhl, V. Vallon, and F. Lang Blunted hypertensive effect of combined fructose and high-salt diet in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase SGK1 Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R935 - R944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. McCormick, V. Bhalla, A. C. Pao, and D. Pearce SGK1: A Rapid Aldosterone-Induced Regulator of Renal Sodium Reabsorption Physiology, April 1, 2005; 20(2): 134 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vallon, P. Wulff, D. Y. Huang, J. Loffing, H. Volkl, D. Kuhl, and F. Lang Role of Sgk1 in salt and potassium homeostasis Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R4 - R10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. de la Rosa, T. G. Paunescu, W. J. Els, S. I. Helman, and C. M. Canessa Mechanisms of Regulation of Epithelial Sodium Channel by SGK1 in A6 Cells J. Gen. Physiol., September 27, 2004; 124(4): 395 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-X. Wang, C. McCrudden, Y.-P. Dai, B. Horowitz, J. R. Hume, and I. A. Yamboliev Hypotonic activation of volume-sensitive outwardly rectifying chloride channels in cultured PASMCs is modulated by SGK Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H533 - H544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markadieu, D. Blero, A. Boom, C. Erneux, and R. Beauwens Phosphatidylinositol 3,4,5-trisphosphate: an early mediator of insulin-stimulated sodium transport in A6 cells Am J Physiol Renal Physiol, August 1, 2004; 287(2): F319 - F328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Palmada, M. Dieter, A. Speil, C. Bohmer, A. F. Mack, H. J. Wagner, K. Klingel, R. Kandolf, H. Murer, J. Biber, et al. Regulation of intestinal phosphate cotransporter NaPi IIb by ubiquitin ligase Nedd4-2 and by serum- and glucocorticoid-dependent kinase 1 Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G143 - G150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Coric, N. Hernandez, D. A. de la Rosa, D. Shao, T. Wang, and C. M. Canessa Expression of ENaC and serum- and glucocorticoid-induced kinase 1 in the rat intestinal epithelium Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G663 - G670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Gumz, M. P. Popp, C. S. Wingo, and B. D. Cain Early transcriptional effects of aldosterone in a mouse inner medullary collecting duct cell line Am J Physiol Renal Physiol, October 1, 2003; 285(4): F664 - F673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. de la Rosa, T. Coric, N. Todorovic, D. Shao, T. Wang, and C. M Canessa Distribution and regulation of expression of serum- and glucocorticoid-induced kinase-1 in the rat kidney J. Physiol., September 1, 2003; 551(2): 455 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing and B. Kaissling Sodium and calcium transport pathways along the mammalian distal nephron: from rabbit to human Am J Physiol Renal Physiol, April 1, 2003; 284(4): F628 - F643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Helms, G. Fejes-Toth, and A. Naray-Fejes-Toth Hormone-regulated transepithelial Na+ transport in mammalian CCD cells requires SGK1 expression Am J Physiol Renal Physiol, March 1, 2003; 284(3): F480 - F487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Maiyar, M. L.L. Leong, and G. L. Firestone Importin-alpha Mediates the Regulated Nuclear Targeting of Serum- and Glucocorticoid-inducible Protein Kinase (Sgk) by Recognition of a Nuclear Localization Signal in the Kinase Central Domain Mol. Biol. Cell, March 1, 2003; 14(3): 1221 - 1239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. A. Itani, K. Z. Liu, K. L. Cornish, J. R. Campbell, and C. P. Thomas Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E971 - E979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nielsen, T.-H. Kwon, S. Masilamani, K. Beutler, H. Hager, S. Nielsen, and M. A. Knepper Sodium transporter abundance profiling in kidney: effect of spironolactone Am J Physiol Renal Physiol, November 1, 2002; 283(5): F923 - F933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Schafer Abnormal regulation of ENaC: syndromes of salt retention and salt wasting by the collecting duct Am J Physiol Renal Physiol, August 1, 2002; 283(2): F221 - F235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Rozansky, J. Wang, N. Doan, T. Purdy, T. Faulk, A. Bhargava, K. Dawson, and D. Pearce Hypotonic induction of SGK1 and Na+ transport in A6 cells Am J Physiol Renal Physiol, July 1, 2002; 283(1): F105 - F113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Stockand New ideas about aldosterone signaling in epithelia Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Urbach and B. J Harvey Rapid and non-genomic reduction of intracellular [Ca2+] induced by aldosterone in human bronchial epithelium J. Physiol., November 15, 2001; 537(1): 267 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Otulakowski, T. Freywald, Y. Wen, and H. O'Brodovich Translational activation and repression by distinct elements within the 5'-UTR of ENaC alpha -subunit mRNA Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1219 - L1231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chigaev, G. Lu, H. Shi, C. Asher, R. Xu, H. Latter, R. Seger, H. Garty, and E. Reuveny In vitro phosphorylation of COOH termini of the epithelial Na+ channel and its effects on channel activity in Xenopus oocytes Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1030 - F1036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Robert-Nicoud, M. Flahaut, J.-M. Elalouf, M. Nicod, M. Salinas, M. Bens, A. Doucet, P. Wincker, F. Artiguenave, J.-D. Horisberger, et al. Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin PNAS, February 15, 2001; (2001) 51603198. [Abstract] [Full Text]
|
|
|
|
|
|
M. Robert-Nicoud, M. Flahaut, J.-M. Elalouf, M. Nicod, M. Salinas, M. Bens, A. Doucet, P. Wincker, F. Artiguenave, J.-D. Horisberger, et al. Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin PNAS, February 27, 2001; 98(5): 2712 - 2716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Faletti, N. Perrotti, S. I. Taylor, and B. L. Blazer-Yost sgk: an essential convergence point for peptide and steroid hormone regulation of ENaC-mediated Na+ transport Am J Physiol Cell Physiol, March 1, 2002; 282(3): C494 - C500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Itani, S. D. Auerbach, R. F. Husted, K. A. Volk, S. Ageloff, M. A. Knepper, J. B. Stokes, and C. P. Thomas Alveolar Epithelial Ion and Fluid Transport: Glucocorticoid-stimulated lung epithelial Na+ transport is associated with regulated ENaC and sgk1 expression Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L631 - L641. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
