AJP - Renal Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 278: F644-F649, 2000;
0363-6127/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mupanomunda, M. M.
Right arrow Articles by Bukoski, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mupanomunda, M. M.
Right arrow Articles by Bukoski, R. D.
Vol. 278, Issue 4, F644-F649, April 2000

Renal interstitial Ca2+

Maria M. Mupanomunda1, Bing Tian1, Norio Ishioka1, and Richard D. Bukoski2

1 Section of Hypertension and Vascular Research, University of Texas Medical Branch, Galveston, Texas 77555-1065 and 2 Julius L. Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, North Carolina 27707

Renal interstitial fluid Ca2+ concentration ([Ca2+]isf) was measured in anesthetized Wistar rats by using in situ microdialysis. During perfusion of 20 cm of the proximal small intestine with Ca2+-free buffer, renal [Ca2+]isf was 1.63 ± 0.19 mmol/l in the cortex (n = 6) and 1.93 ± 0.12 mmol/l in the medulla (n = 5, P = 0.223). When Ca2+ in the intestinal lumen was increased to 3 mmol/l, no change was seen in total or ionized serum Ca2+ (SCa), urinary Ca2+ excretion (UCa), or Ca2+ in a microdialysate of the kidney cortex. Increasing intestinal Ca2+ further, to 6 mmol/l, was without effect on SCa but significantly increased UCa by 38% and microdialysate Ca2+ by 36% (1.25 ± 0.0.09 vs. 1.70 ± 0.14 mmol/l, n = 4, P < 0.05). Intravenous infusion of 28 ng · kg-1 · min-1 of parathyroid hormone for 1 h during perfusion of the intestinal lumen with 1 mmol/ Ca2+caused a 7-10% rise in SCa, a 40% fall in UCa, and a 32% increase in microdialysate Ca2+ (1.32 ± 0.13 vs. 1.74 ± 0.13 mmol/l, n = 6, P < 0.05). Interlobar arteries with a mean diameter of 120 µm were studied by using a wire myograph to determine whether changes in extracellular Ca2+ affect muscle tone. When precontracted with 5 µmol/l serotonin, the arteries relaxed in response to cumulative addition of Ca2+ (1-5 mmol/l) with an ED50 value for Ca2+ of 3.30 ± 0.08 mmol/l, n = 3. These data demonstrate that [Ca2+]isf changes dynamically during manipulation of whole-animal Ca2+ homeostasis and that intrarenal arteries relax in response to extracellular Ca2+ varied over the range measured in vivo.

kidney; calcium; microdialysis; relaxation


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. C. Ortiz-Capisano, T.-D. Liao, P. A. Ortiz, and W. H. Beierwaltes
Calcium-dependent phosphodiesterase 1C inhibits renin release from isolated juxtaglomerular cells
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2009; 297(5): R1469 - R1476.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. Caroppo, A. Gerbino, G. Fistetto, M. Colella, L. Debellis, A. M. Hofer, and S. Curci
Extracellular calcium acts as a "third messenger" to regulate enzyme and alkaline secretion
J. Cell Biol., July 5, 2004; 166(1): 111 - 119.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
G Z Racz, A Kittel, D Riccardi, R M Case, A C Elliott, and G Varga
Extracellular calcium sensing receptor in human pancreatic cells
Gut, November 1, 2002; 51(5): 705 - 711.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. D. Bukoski, S. Shearin, W. F. Jackson, and M. F. Pamarthi
Inhibition of Ca2+-Induced Relaxation by Oxidized Tungsten Wires and Paratungstate
J. Pharmacol. Exp. Ther., October 1, 2001; 299(1): 343 - 350.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
R. D. Bukoski
Dietary Ca2+ and blood pressure: evidence that Ca2+-sensing receptor activated, sensory nerve dilator activity couples changes in interstitial Ca2+ with vascular tone
Nephrol. Dial. Transplant., February 1, 2001; 16(2): 218 - 221.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online