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Am J Physiol Renal Physiol 278: F689-F701, 2000;
0363-6127/00 $5.00
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Vol. 278, Issue 5, F689-F701, May 2000

INVITED REVIEW
Pushing, pulling, dragging, and vibrating renal epithelia by using atomic force microscopy

Robert M. Henderson1 and Hans Oberleithner2

1 Department of Pharmacology, University of Cambridge, Cambridge CB2 1QJ, United Kingdom; and 2 Institut für Physiologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany

Renal physiologists focus on events that take place on and around the surfaces of cells. Various techniques have been developed that follow transport functions at the molecular level, but until recently none of these techniques has been capable of making the behavior of molecular structures visible under physiological conditions. This apparent gap may be filled in the future by the application of atomic force microscopy. This technique produces an image not by optical means, but by "feeling" its way across a surface. Atomic force microscopy can, however, be modified in a number of ways, which means that besides producing a high-resolution image, it is possible to obtain several types of data on the interactions between the ultrastructural components of cell membranes (such as proteins) and other biologically active molecules (such as ATP). In this review we describe the recent use of the atomic force microscope in renal physiology, ranging from experiments in intact cells to those in isolated renal transport protein molecules, include examples of these extended applications of the technique, and point to uses that the microscope has recently found in other areas of biology that should prove fruitful in renal physiology in the near future.

kidney; scanning probe microspcopy; Madin-Darby canine kidney cells; ion channels; nuclear membrane; plasma membrane


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