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Am J Physiol Renal Physiol 278: F737-F746, 2000;
0363-6127/00 $5.00
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Vol. 278, Issue 5, F737-F746, May 2000

Similarities and differences in the subcellular localization of hamartin and tuberin in the kidney

Vanishree Murthy1,*, Luciana A. Haddad1,*, Nicole Smith1, Denise Pinney1, Robert Tyszkowski2, Dennis Brown2, and Vijaya Ramesh1

1 Molecular Neurogenetics Unit and 2 Renal Unit Massachusetts General Hospital, Charlestown, Massachusetts 02129

Tuberous sclerosis complex (TSC) is an autosomal dominant disorder characterized by hamartomas in multiple organs, notably the brain and kidneys. The disease is caused by mutations in TSC1 or TSC2 genes, coding hamartin and tuberin, respectively. Immunofluorescence analysis of tuberin and hamartin performed here demonstrates that both proteins are specifically expressed in the distal urinary tubule, comprising the distal tubules, connecting segment, and collecting ducts. Hamartin, distinct from tuberin, is expressed in the thick ascending limbs of Henle and in juxtaglomerular cells, where it colocalizes with renin. In positive epithelial cells, tuberin localizes to the cytoplasm as well as the apical membrane. Hamartin, however, preferentially localizes to the apical membrane. The two proteins colocalize at the apical membrane of type A intercalated cells and connecting tubule cells, whereas in type B intercalated cells they reveal a variable pattern of expression. The cell-specific expression of tuberin and hamartin described here will provide critical insight into the cell types that give rise to kidney lesions, and the tumor suppressor role of these proteins in TSC.

tuberous sclerosis; hamartomas; intercalated cells; apical membrane

* V. Murthy and L. A. Haddad contributed equally to this work.   The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.




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