| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Nephrology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205
Tonicity-responsive enhancer binding protein (TonEBP) regulates transcription of tonicity responsive genes such as the sodium-myo-inositol cotransporter (SMIT), the sodium-chloride-betaine cotransporter (BGT1), and aldose reductase (AR). To characterize signals that activate TonEBP in Madin-Darby canine kidney (MDCK) cells, the abundance and nuclear distribution of TonEBP were studied after the osmolality of the culture medium was changed. Hypertonicity but not hyperosmolality is effective in activation of TonEBP as expected. Surprisingly, exposure to hypotonic medium leads to a dramatic downregulation of TonEBP both in abundance and nuclear distribution, indicating that under isotonic conditions, TonEBP is at a low-level activated state and can respond to both increase and decrease in tonicity. Additional experiments suggest that cellular ionic strength is the signal that initiates regulation of TonEBP. The increase in abundance of TonEBP is mediated by an increase in mRNA abundance and a parallel increase in synthesis of TonEBP. The stability of TonEBP mRNA is not affected by hypertonicity indicating that transcription plays a major role in the induction of TonEBP by hypertonicity.
hypertonicity-stimulated transcription; sodium-myo-inositol cotransporter; sodium-chloride-betaine cotransporter; aldose reductase
This article has been cited by other articles:
|
|
 |
|
  M. A. Lanaspa, A. Andres-Hernando, C. J. Rivard, Y. Dai, N. Li, and T. Berl ZAC1 Is Up-regulated by Hypertonicity and Decreases Sorbitol Dehydrogenase Expression, Allowing Accumulation of Sorbitol in Kidney Cells J. Biol. Chem., July 24, 2009; 284(30): 19974 - 19981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Kwon, S. W. Lim, and H. M. Kwon Hypertonic Stress in the Kidney: A Necessary Evil Physiology, June 1, 2009; 24(3): 186 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Hasler Controlled aquaporin-2 expression in the hypertonic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C641 - C653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen Physiology of Cell Volume Regulation in Vertebrates Physiol Rev, January 1, 2009; 89(1): 193 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Kim, W.-Y. Kim, H.-W. Lee, J. Kim, H. M. Kwon, J. D. Klein, J. M. Sands, and D. Kim Urea and NaCl regulate UT-A1 urea transporter in opposing directions via TonEBP pathway during osmotic diuresis Am J Physiol Renal Physiol, January 1, 2009; 296(1): F67 - F77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rodgaard, K. Schou, M. B. Friis, and E. K. Hoffmann Does the intracellular ionic concentration or the cell water content (cell volume) determine the activity of TonEBP in NIH3T3 cells? Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1528 - C1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Saito, T. Saito, K. Kasono, H. Tamemoto, M. Kawakami, S. Sasaki, and S.-e Ishikawa Hypotonicity reduces the activity of murine aquaporin-2 promoter induced by dibutyryl cAMP Exp Physiol, October 1, 2008; 93(10): 1147 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bissonnette, K. Lahjouji, M. J. Coady, and J.-Y. Lapointe Effects of hyperosmolarity on the Na+-myo-inositol cotransporter SMIT2 stably transfected in the Madin-Darby canine kidney cell line Am J Physiol Cell Physiol, September 1, 2008; 295(3): C791 - C799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Sazonova, K. A. James, C. R. McCudden, D. Segal, A. Talebian, and G. F. Wagner Stanniocalcin-1 secretion and receptor regulation in kidney cells Am J Physiol Renal Physiol, April 1, 2008; 294(4): F788 - F794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Kelley and B. M. Paschal Hyperosmotic Stress Signaling to the Nucleus Disrupts the Ran Gradient and the Production of RanGTP Mol. Biol. Cell, November 1, 2007; 18(11): 4365 - 4376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Burg, J. D. Ferraris, and N. I. Dmitrieva Cellular Response to Hyperosmotic Stresses Physiol Rev, October 1, 2007; 87(4): 1441 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kosuge, A. I. Chuang, S. Uematsu, K. P. Tan, K. Ohashi, B. C.B. Ko, and S. Ito Discovery of Osmosensitive Transcriptional Regulation of Human Cytochrome P450 3As by the Tonicity-Responsive Enhancer Binding Protein (Nuclear Factor of Activated T Cells 5) Mol. Pharmacol., October 1, 2007; 72(4): 826 - 837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. S. Jeon, K.-H. Han, S.-H. Park, S. D. Lee, M. R. Sheen, J.-Y. Jung, W. Y. Kim, J. M. Sands, J. Kim, and H. M. Kwon Downregulation of renal TonEBP in hypokalemic rats Am J Physiol Renal Physiol, July 1, 2007; 293(1): F408 - F415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, M. P. Schnetz, C. E. Irarrazabal, R.-F. Shen, C. K. Williams, M. B. Burg, and J. D. Ferraris Proteomic identification of proteins associated with the osmoregulatory transcription factor TonEBP/OREBP: functional effects of Hsp90 and PARP-1 Am J Physiol Renal Physiol, March 1, 2007; 292(3): F981 - F992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Lim, K. O. Ahn, M. R. Sheen, U. S. Jeon, J. Kim, C. W. Yang, and H. M. Kwon Downregulation of Renal Sodium Transporters and Tonicity-Responsive Enhancer Binding Protein by Long-Term Treatment with Cyclosporin A J. Am. Soc. Nephrol., February 1, 2007; 18(2): 421 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. O'Connor, S. T. Mills, K. A. Jones, S. N. Ho, and G. K. Pavlath A combinatorial role for NFAT5 in both myoblast migration and differentiation during skeletal muscle myogenesis J. Cell Sci., January 1, 2007; 120(1): 149 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, U. S. Jeon, J. A. Kim, D. Mordasini, H. M. Kwon, E. Feraille, and P.-Y. Martin Tonicity-Responsive Enhancer Binding Protein Is an Essential Regulator of Aquaporin-2 Expression in Renal Collecting Duct Principal Cells J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1521 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, A. D. Hodgkinson, P. J. Oates, H. M. Kwon, B. A. Millward, and A. G. Demaine Elevated activity of transcription factor nuclear factor of activated T-cells 5 (NFAT5) and diabetic nephropathy. Diabetes, May 1, 2006; 55(5): 1450 - 1455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Cai, J. D. Ferraris, and M. B. Burg High NaCl increases TonEBP/OREBP mRNA and protein by stabilizing its mRNA Am J Physiol Renal Physiol, October 1, 2005; 289(4): F803 - F807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kultz DNA damage signals facilitate osmotic stress adaptation Am J Physiol Renal Physiol, September 1, 2005; 289(3): F504 - F505. [Full Text] [PDF]
|
|
|
|
|
|
N. I. Dmitrieva, M. B. Burg, and J. D. Ferraris DNA damage and osmotic regulation in the kidney Am J Physiol Renal Physiol, July 1, 2005; 289(1): F2 - F7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Han, S. K. Woo, W.-Y. Kim, S.-H. Park, J.-H. Cha, J. Kim, and H. M. Kwon Maturation of TonEBP expression in developing rat kidney Am J Physiol Renal Physiol, November 1, 2004; 287(5): F878 - F885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Cai, J. D. Ferraris, and M. B. Burg Greater tolerance of renal medullary cells for a slow increase in osmolality is associated with enhanced expression of HSP70 and other osmoprotective genes Am J Physiol Renal Physiol, January 1, 2004; 286(1): F58 - F67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Lee, E. Colla, M. R. Sheen, K. Y. Na, and H. M. Kwon Multiple Domains of TonEBP Cooperate to Stimulate Transcription in Response to Hypertonicity J. Biol. Chem., November 28, 2003; 278(48): 47571 - 47577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Kempson, V. Parikh, L. Xi, S. Chu, and M. H. Montrose Subcellular redistribution of the renal betaine transporter during hypertonic stress Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1091 - C1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, J. D. Ferraris, H. L. Brooks, I. Brisc, and M. B. Burg Expression of osmotic stress-related genes in tissues of normal and hyposmotic rats Am J Physiol Renal Physiol, October 1, 2003; 285(4): F688 - F693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Na, S. K. Woo, S. D. Lee, and H. M. Kwon Silencing of TonEBP/NFAT5 Transcriptional Activator by RNA Interference J. Am. Soc. Nephrol., February 1, 2003; 14(2): 283 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Storm, E. Klussmann, A. Geelhaar, W. Rosenthal, and K. Maric Osmolality and solute composition are strong regulators of AQP2 expression in renal principal cells Am J Physiol Renal Physiol, January 1, 2003; 284(1): F189 - F198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Neuhofer, S. K. Woo, K. Y. Na, R. Grunbein, W. K. Park, O. Nahm, F.-X. Beck, and H. M. Kwon Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1604 - C1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Srivastava, B. Chandrasekar, A. Bhatnagar, and S. D. Prabhu Lipid peroxidation-derived aldehydes and oxidative stress in the failing heart: role of aldose reductase Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2612 - H2619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Woo, S. D. Lee, K. Y. Na, W. K. Park, and H. M. Kwon TonEBP/NFAT5 Stimulates Transcription of HSP70 in Response to Hypertonicity Mol. Cell. Biol., August 15, 2002; 22(16): 5753 - 5760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Soupart, S. Silver, B. Schrooeder, R. Sterns, and G. Decaux Rapid (24-Hour) Reaccumulation of Brain Organic Osmolytes (Particularly myo-Inositol) in Azotemic Rats after Correction of Chronic Hyponatremia J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1433 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. CHA, S. K. WOO, K. H. HAN, Y. H. KIM, J. S. HANDLER, J. KIM, and H. M. KWON Hydration Status Affects Nuclear Distribution of Transcription Factor Tonicity Responsive Enhancer Binding Protein in Rat Kidney J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2221 - 2230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Franchi-Gazzola, R. Visigalli, V. Dall'Asta, R. Sala, S. K. Woo, H. M. Kwon, G. C. Gazzola, and O. Bussolati Amino acid depletion activates TonEBP and sodium-coupled inositol transport Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1465 - C1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Tian and D. M. Cohen Urea inhibits hypertonicity-inducible TonEBP expression and action Am J Physiol Renal Physiol, May 1, 2001; 280(5): F904 - F912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Ferraris, C. K. Williams, P. Persaud, Z. Zhang, Y. Chen, and M. B. Burg Activity of the TonEBP/OREBP transactivation domain varies directly with extracellular NaCl concentration PNAS, January 22, 2002; 99(2): 739 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Nahm, S. K. Woo, J. S. Handler, and H. M. Kwon Involvement of multiple kinase pathways in stimulation of gene transcription by hypertonicity Am J Physiol Cell Physiol, January 1, 2002; 282(1): C49 - C58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Maouyo, J. Y. Kim, S. D. Lee, Y. Wu, S. K. Woo, and H. M. Kwon Mouse TonEBP-NFAT5: expression in early development and alternative splicing Am J Physiol Renal Physiol, May 1, 2002; 282(5): F802 - F809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhao, W. Tian, and D. M. Cohen Rottlerin inhibits tonicity-dependent expression and action of TonEBP in a PKCdelta -independent fashion Am J Physiol Renal Physiol, April 1, 2002; 282(4): F710 - F717. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
