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Am J Physiol Renal Physiol 278: F894-F904, 2000;
0363-6127/00 $5.00
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Vol. 278, Issue 6, F894-F904, June 2000

Carbonic anhydrase IV is expressed in H+-secreting cells of rabbit kidney

George J. Schwartz1,2, Ann M. Kittelberger1, Darlene A. Barnhart1, and Soundarapandian Vijayakumar3

1 Departments of Pediatrics and Medicine and 2 Division of Pediatric Nephrology, University of Rochester School of Medicine, Rochester 14642; and 3 Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, New York 10032

Carbonic anhydrase (CA) IV is a membrane-bound enzyme that catalyzes the dehydration of carbonic acid to CO2 and water. Using peptides from each end of the deduced rabbit CA IV amino acid sequence, we generated a goat anti-rabbit CA IV antibody, which was used for immunoblotting and immunohistochemical analysis. CA IV was expressed in a variety of organs including spleen, heart, lung, skeletal muscle, colon, and kidney. Rabbit kidney CA IV had two N-glycosylation sites and was sialated, the apparent molecular mass increasing by at least 11 to ~45 kDa in the cortex. Medullary CA IV was much more heavily glycosylated than CA IV from cortex or any other organ, such modifications increasing the molecular mass by at least 20 kDa. CA IV was expressed on the apical and basolateral membranes of proximal tubules with expression levels on the order of S2 > S1 > S3 = 0. Because CA IV is believed to be anchored to the apical membrane by glycosylphosphatidylinositol, the presence of basolateral CA IV suggests an alternative mechanism. CA IV was localized on the apical membranes of outer medullary collecting duct cells of the inner stripe and inner medullary collecting duct cells, as well as on alpha -intercalated cells. However, CA IV was not expressed by beta -intercalated cells, glomeruli, distal tubule, or Henle's loop cells. Thus CA IV was expressed by H+-secreting cells of the rabbit kidney, suggesting an important role for CA IV in urinary acidification.

organs; intercalated cells; proximal tubule; medullary collecting duct; immunohistochemistry; Western blot


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