| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C; 2 Department of Clinical Physiology, Aarhus University Hospital and Institute of Experimental Clinical Research, DK-8200 Aarhus N, Denmark; 3 Department of Internal Medicine, Seoul National University Hospital, 110-744 Seoul, Korea; and 4 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
Ischemia-induced acute renal failure (ARF) is known to be associated with significant impairment of tubular Na reabsorption. We examined whether temporary bilateral renal ischemia (30, 40, or 60 min) and reperfusion (1-5 days) affect the abundance of several renal Na transporters and urinary Na excretion (UNaV) in rats. In rats with mild ARF (30 min), immunoblotting revealed that proximal tubule type 3 Na+/H+ exchanger (NHE-3) and type II Na-Pi cotransporter (NaPi-II) were significantly decreased to 28 ± 6 and 14 ± 6% of sham levels, respectively, at day 1. Moreover, Na+-K+-ATPase levels were also significantly decreased (51 ± 11%), whereas there was no significant decrease in type 1 bumetanide-sensitive cotransporter (BSC-1) and thiazide-sensitive cotransporter (TSC) levels. Consistent with reduced Na transporter abundance, fractional urinary Na excretion (FENa) was significantly increased in mild ARF (30 min) and UNaV was unchanged, despite a marked reduction in glomerular filtration rate. Na transporter levels and renal Na handling were normalized within 5 days. Severe ischemic injury (60 min) resulted in a marked decrease in the abundance of Na+-K+-ATPase, NHE-3, NaPi-II, BSC-1, and TSC at both days 1 and 5. Consistent with this, FENa was significantly increased at days 1 and 5. Intravenous K-melanocyte-stimulated hormone treatment partially prevented the ischemia-induced downregulation of renal Na transporters and reduced the high FENa to control levels. We conclude that reduced levels of Na transporters along the nephron may play a critical role in the impairment of tubular Na reabsorption, and hence increased Na excretion, in ischemia-induced ARF.
sodium transport; sodium-hydrogen exchanger; sodium-inorganic phosphate cotransporter; sodium-potassium-2 chloride transporter; sodium-chloride cotransporter
This article has been cited by other articles:
|
|
 |
|
  S. G. Coca and C. R. Parikh Urinary Biomarkers for Acute Kidney Injury: Perspectives on Translation Clin. J. Am. Soc. Nephrol., March 1, 2008; 3(2): 481 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-H. Han, H.-Y. Kim, B. P. Croker, S. Reungjui, S.-Y. Lee, J. Kim, M. E. Handlogten, C. A. Adin, and I. D. Weiner Effects of ischemia-reperfusion injury on renal ammonia metabolism and the collecting duct Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1342 - F1354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Schmidt, K. Hocherl, F. Schweda, A. Kurtz, and M. Bucher Regulation of Renal Sodium Transporters during Severe Inflammation J. Am. Soc. Nephrol., April 1, 2007; 18(4): 1072 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. de Seigneux, H. Malte, H. Dimke, J. Frokiaer, S. Nielsen, and S. Frische Renal compensation to chronic hypoxic hypercapnia: downregulation of pendrin and adaptation of the proximal tubule Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1256 - F1266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Andrade, A. C. Rodrigues Jr., T. R. C. Sanches, R. B. Souza, and A. C. Seguro Leptospirosis leads to dysregulation of sodium transporters in the kidney and lung Am J Physiol Renal Physiol, February 1, 2007; 292(2): F586 - F592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fekete, A. Vannay, A. Ver, K. Rusai, V. Muller, G. Reusz, T. Tulassay, and A. J. Szabo Sex differences in heat shock protein 72 expression and localization in rats following renal ischemia-reperfusion injury Am J Physiol Renal Physiol, October 1, 2006; 291(4): F806 - F811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. N. Lee, E. Huang, and H. J. Ward Tight junction biology and kidney dysfunction Am J Physiol Renal Physiol, January 1, 2006; 290(1): F20 - F34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Runembert, S. Couette, P. Federici, E. Colucci-Guyon, C. Babinet, P. Briand, G. Friedlander, and F. Terzi Recovery of Na-glucose cotransport activity after renal ischemia is impaired in mice lacking vimentin Am J Physiol Renal Physiol, November 1, 2004; 287(5): F960 - F968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Velic, J. R. Hirsch, J. Bartel, R. Thomas, R. Schroter, H. Stegemann, B. Edemir, C. August, E. Schlatter, and G. Gabriels Renal Transplantation Modulates Expression and Function of Receptors and Transporters of Rat Proximal Tubules J. Am. Soc. Nephrol., April 1, 2004; 15(4): 967 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Catania, S. Gatti, G. Colombo, and J. M. Lipton Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation Pharmacol. Rev., March 1, 2004; 56(1): 1 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fekete, A. Vannay, A. Ver, B. Vasarhelyi, V. Muller, N. Ouyang, G. Reusz, T. Tulassay, and A. J. Szabo Sex differences in the alterations of Na+,K+-ATPase following ischaemia-reperfusion injury in the rat kidney J. Physiol., March 1, 2004; 555(2): 471 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Graebe, L. Brond, S. Christensen, S. Nielsen, N. V. Olsen, and T. E. N. Jonassen Chronic nitric oxide synthase inhibition exacerbates renal dysfunction in cirrhotic rats Am J Physiol Renal Physiol, February 1, 2004; 286(2): F288 - F297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gong, W. Wang, T.-H. Kwon, T. Jonassen, J. Frokiaer, and S. Nielsen Reduced renal expression of AQP2, p-AQP2 and AQP3 in haemorrhagic shock-induced acute renal failure Nephrol. Dial. Transplant., December 1, 2003; 18(12): 2551 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Rajasekaran and S. A. Rajasekaran Role of Na-K-ATPase in the assembly of tight junctions Am J Physiol Renal Physiol, September 1, 2003; 285(3): F388 - F396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Wald, Y. Figueroa, A. S. Oriolo, and P. J. I. Salas Membrane repolarization is delayed in proximal tubules after ischemia-reperfusion: possible role of microtubule-organizing centers Am J Physiol Renal Physiol, August 1, 2003; 285(2): F230 - F240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Bek, H. C. Reinhardt, K.-G. Fischer, J. R. Hirsch, C. Hupfer, E. Dayal, and H. Pavenstadt Up-Regulation of Early Growth Response Gene-1 Via the CXCR3 Receptor Induces Reactive Oxygen Species and Inhibits Na+/K+-ATPase Activity in an Immortalized Human Proximal Tubule Cell Line J. Immunol., January 15, 2003; 170(2): 931 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiar Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction Am J Physiol Renal Physiol, January 1, 2003; 284(1): F155 - F166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-L. Elkjar, T.-H. Kwon, W. Wang, J. Nielsen, M. A. Knepper, J. Frokiar, and S. Nielsen Altered expression of renal NHE3, TSC, BSC-1, and ENaC subunits in potassium-depleted rats Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1376 - F1388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. N. Jonassen, M. Grabe, D. Promeneur, S. Nielsen, S. Christensen, and N. V. Olsen Lipopolysaccharide-Induced Acute Renal Failure in Conscious Rats: Effects of Specific Phosphodiesterase Type 3 and 4 Inhibition J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 364 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Knepper Proteomics and the Kidney J. Am. Soc. Nephrol., May 1, 2002; 13(5): 1398 - 1408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Boyd-White, A. Srirangam, M. P. Goheen, and M. C. Wagner Ischemia disrupts myosin Ibeta in renal tubules Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1326 - C1335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, T.-H. Kwon, C. Li, A. Flyvbjerg, M. A. Knepper, J. Frokiar, and S. Nielsen Altered expression of renal aquaporins and Na+ transporters in rats treated with L-type calcium blocker Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1632 - R1641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Nejsum, T.-H. Kwon, D. Marples, A. Flyvbjerg, M. A. Knepper, J. Frokiar, and S. Nielsen Compensatory increase in AQP2, p-AQP2, and AQP3 expression in rats with diabetes mellitus Am J Physiol Renal Physiol, April 1, 2001; 280(4): F715 - F726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, T.-H. Kwon, C. Li, J. Frokiar, M. A. Knepper, and S. Nielsen Reduced expression of Na-K-2Cl cotransporter in medullary TAL in vitamin D-induced hypercalcemia in rats Am J Physiol Renal Physiol, January 1, 2002; 282(1): F34 - F44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Narkar, T. Hussain, and M. Lokhandwala Role of tyrosine kinase and p44/42 MAPK in D2-like receptor-mediated stimulation of Na+, K+-ATPase in kidney Am J Physiol Renal Physiol, April 1, 2002; 282(4): F697 - F702. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
