AJP - Renal Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 278: F999-F1005, 2000;
0363-6127/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gloy, J.
Right arrow Articles by Pavenstädt, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gloy, J.
Right arrow Articles by Pavenstädt, H.
Vol. 278, Issue 6, F999-F1005, June 2000

Amino acid transport in podocytes

Joachim Gloy1, Steffen Reitinger1, Karl-Georg Fischer1, Rainer Schreiber2, Anissa Boucherot2, Karl Kunzelmann2, Peter Mundel3, and Hermann Pavenstädt1

1 Department of Medicine, Division of Nephrology, and 2 Department of Physiology, Albert-Ludwigs-University Freiburg, D-79106 Freiburg, Germany; and 3 Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York

It has recently been shown that formation of podocyte foot processes is dependent on a constant source of lipids and proteins (Simons M, Saffrich R, Reiser J, and Mundel P. J Am Soc Nephrol 10: 1633-1639, 1999). Here we characterize amino acid transport mechanisms in differentiated cultured podocytes and investigate whether it may be disturbed during podocyte injury. RT-PCR studies detected mRNA for transporters of neutral amino acids (ASCT1, ASCT2, and B0/+), cationic AA (CAT1 and CAT3), and anionic AA (EAAT2 and EAAT3). Alanine (Ala), asparagine, cysteine (Cys), glutamine (Gln), glycine (Gly), leucine (Leu), methionine (Met), phenylalanine (Phe), proline (Pro), serine (Ser), threonine (Thr), glutamic acid (Glu), arginine (Arg), and histidine (His) depolarized podocytes and increased their whole cell conductances. Depletion of extracellular Na+ completely inhibited the depolarization induced by Ala, Gln, Glu, Gly, Leu, and Pro and decreased the depolarization induced by Arg and His, indicating the presence of Na+-dependent amino acid transport. Incubation of podocytes with 100 µg/ml puromycin aminonucleoside for 24 h significantly attenuated the effects induced by the various amino acids by ~70%. The data indicate the existence of different amino acid transporter systems in podocytes. Alteration of amino acid transport may participate in podocyte injury and disturbed foot process formation.

podocytes; amino acid transport; puromycin


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
Y. Sekine, Y. Nishibori, Y. Akimoto, A. Kudo, N. Ito, D. Fukuhara, R. Kurayama, E. Higashihara, E. Babu, Y. Kanai, et al.
Amino Acid Transporter LAT3 Is Required for Podocyte Development and Function
J. Am. Soc. Nephrol., July 1, 2009; 20(7): 1586 - 1596.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. P. Rastaldi, S. Armelloni, S. Berra, N. Calvaresi, A. Corbelli, L. A. Giardino, M. Li, G. Q. Wang, A. Fornasieri, A. Villa, et al.
Glomerular podocytes contain neuron-like functional synaptic vesicles
FASEB J, May 1, 2006; 20(7): 976 - 978.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Rico, A. Mukherjee, M. Konieczkowski, L. A. Bruggeman, R. T. Miller, S. Khan, J. R. Schelling, and J. R. Sedor
WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F431 - F441.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. D. Cohen, P. P. Doran, S. M. Blattner, M. Merkle, G. Q. Wang, H. Schmid, P. W. Mathieson, M. A. Saleem, A. Henger, M. P. Rastaldi, et al.
Sam68-Like Mammalian Protein 2, Identified by Digital Differential Display as Expressed by Podocytes, Is Induced in Proteinuria and Involved in Splice Site Selection of Vascular Endothelial Growth Factor
J. Am. Soc. Nephrol., July 1, 2005; 16(7): 1958 - 1965.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. J. Morton, K. Hutchinson, P. W. Mathieson, I. R. Witherden, M. A. Saleem, and M. Hunter
Human Podocytes Possess a Stretch-Sensitive, Ca2+-Activated K+ Channel: Potential Implications for the Control of Glomerular Filtration
J. Am. Soc. Nephrol., December 1, 2004; 15(12): 2981 - 2987.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. Pavenstadt, W. Kriz, and M. Kretzler
Cell Biology of the Glomerular Podocyte
Physiol Rev, January 1, 2003; 83(1): 253 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
A. Boucherot, R. Schreiber, H. Pavenstadt, and K. Kunzelmann
Cloning and expression of the mouse glomerular podoplanin homologue gp38P
Nephrol. Dial. Transplant., June 1, 2002; 17(6): 978 - 984.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online