AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 279: F102-F111, 2000;
0363-6127/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rubera, I.
Right arrow Articles by Poujeol, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rubera, I.
Right arrow Articles by Poujeol, P.
Vol. 279, Issue 1, F102-F111, July 2000

Extracellular ATP increases [Ca2+]i in distal tubule cells. II. Activation of a Ca2+-dependent Clminus conductance

Isabelle Rubera, Michel Tauc, Michel Bidet, Catherine Verheecke-Mauze, Guy De Renzis, Chantal Poujeol, Béatrice Cuiller, and Philippe Poujeol

Unité Mixte de Recherche 6548, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, 06108 Nice Cedex 2, France

We characterized Cl- conductance activated by extracellular ATP in an immortalized cell line derived from rabbit distal bright convoluted tubule (DC1). 125I- efflux experiments showed that ATP increased 125I- loss with an EC50 = 3 µM. Diphenylamine-2-carboxylate (10-3 M) and NPPB (10-4 M) abolished the 125I- efflux. Preincubation with 10 µM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester or 10-7 M thapsigargin inhibited the effect of ATP. Ionomycin (2 µM) increased 125I- efflux with a time course similar to that of extracellular ATP, suggesting that the response is dependent on the intracellular Ca2+ concentration ([Ca2+]i). The ATP agonist potency order was ATP >=  UTP > ATPgamma S. Suramin (500 µM) inhibited the ATP-induced 125I- efflux, consistent with P2 purinoceptors. 125I- effluxes from cells grown on permeable filters suggest that ATP induced an apical efflux that was mediated via apical P2 receptors. Whole cell experiments showed that ATP (100 µM) activated outwardly rectifying Cl- currents in the presence of 8-cyclopentyl-1,3-dipropylxanthine, excluding the involvement of P1 receptors. Ionomycin activated Cl- currents similar to those developed with ATP. These results demonstrate the presence of a purinergic regulatory mechanism involving ATP, apical P2Y2 receptors, and Ca2+ mobilization for apical Cl- conductance in a distal tubule cell line.

kidney; intracellular calcium


This article has been cited by other articles:


Home page
 PhysiologyHome page
R. J. Unwin, M. A. Bailey, and G. Burnstock
Purinergic Signaling Along the Renal Tubule: The Current State of Play
Physiology, December 1, 2003; 18(6): 237 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. S. Wildman, K. M. Hooper, C. M. Turner, J. S. K. Sham, E. G. Lakatta, B. F. King, R. J. Unwin, and M. Sutters
The isolated polycystin-1 cytoplasmic COOH terminus prolongs ATP-stimulated Cl- conductance through increased Ca2+ entry
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1168 - F1178.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. B. Woda, M. Leite Jr., R. Rohatgi, and L. M. Satlin
Effects of luminal flow and nucleotides on [Ca2+]i in rabbit cortical collecting duct
Am J Physiol Renal Physiol, September 1, 2002; 283(3): F437 - F446.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. H. Clarson, V. H. J. Roberts, S. L. Greenwood, and A. C. Elliott
ATP-stimulated Ca2+-activated K+ efflux pathway and differentiation of human placental cytotrophoblast cells
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1077 - R1085.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. W. Inscho
P2 receptors in regulation of renal microvascular function
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F927 - F944.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. M. Schwiebert and B. K. Kishore
Extracellular nucleotide signaling along the renal epithelium
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F945 - F963.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Rubera, H. Barriere, M. Tauc, M. Bidet, C. Verheecke-Mauze, C. Poujeol, B. Cuiller, and P. Poujeol
Extracellular adenosine modulates a volume-sensitive-like chloride conductance in immortalized rabbit DC1 cells
Am J Physiol Renal Physiol, January 1, 2001; 280(1): F126 - F145.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online