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Departments of 1 Physiology and 2 Nephrology, Göteborg University, SE-405 30 Göteborg, Sweden
The fractional clearances (
) for
FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys
in which the tubular activity was inhibited by low temperature (8°C)
and/or 10 mM NH4Cl. The Ficoll data were analyzed according
to a two-pore model giving small and large pore radii of 46 Å and
80-87 Å, respectively. The estimated negative charge density was
35-45 meq/l at 8°C. Perfusion with erythrocyte-free solutions of
kidneys at 37°C reduced glomerular size and charge
permselectivity. Thus the large pore fraction of the glomerular
filtrate (fL) was 1.64% at 37°C compared with 0.94% at
8°C. The for albumin was four times higher at 37°C than at
8°C (0.86% vs. 0.19%, respectively). NH4Cl caused further irreversible damage to the glomerular barrier. We conclude that
there are no deleterious effects on the glomerular barrier of a
reduction in temperature from 37°C to 8°C. Therefore our data seem
to disprove the hypothesis of low glomerular permselectivity and
transtubular uptake of intact albumin and support the classic concept of a highly selective glomerular barrier.
capillary permeability; macromolecular transport; two-pore model
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