AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 279: F243-F251, 2000;
0363-6127/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ingram, A. J.
Right arrow Articles by Scholey, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ingram, A. J.
Right arrow Articles by Scholey, J. W.
Vol. 279, Issue 2, F243-F251, August 2000

Nitric oxide modulates mechanical strain-induced activation of p38 MAPK in mesangial cells

A. J. Ingram1, L. James2, K. Thai2, H. Ly2, L. Cai2, and J. W. Scholey2

1 Department of Medicine, McMaster University, Hamilton, Ontario L8N 1Y2; and 2 University of Toronto, Toronto, Ontario, Canada M55 1A8

Mesangial cells (MC), grown on extracellular matrix (ECM) protein-coated plates and stretched, proliferate and produce ECM, recapitulating in vivo responses to increased glomerular capillary pressure (Pgc). Transduction of strain involves mitogen-activated protein kinases (MAPK), and we have shown that p38 MAPK is activated by strain in MC. Because in vivo studies show that nitric oxide (NO) in the remnant kidney limits glomerular injury without reducing Pgc, we studied whether NO attenuated stretch-induced p38 activation in MC. Increasing p38 activation occurred with increasing stretch, maximally at 10 min at -27-kPa vacuum. Cyclic strain increased nuclear translocation of phosphorylated p38 by immunofluorescent microscopy and nuclear protein binding to nuclear factor-kappa B (NF-kappa B) consensus sequences by mobility shift assay. Both events were largely abrogated by the p38 inhibitor SB-203580. The NO donors 3-morpholinosydnonimine, S-nitroso-N-acetylpenicillamine, and 8-bromoguanosine 3',5'-cyclic monophosphate, a stable cGMP analog, prevented p38 activation and nulcear translocation. Thus strain induces p38 activity and translocation to the nucleus and p38-dependent increases in nuclear protein binding to NF-kappa B. This pathway is attenuated by the NO donors or a cGMP analog.

nuclear factor-kappa B; kidney glomerulus; physical forces; mitogen-activated protein kinases; signaling


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
L. C. Martineau, L. I. McVeigh, B. J. Jasmin, and C. R. J. Kennedy
p38 MAP kinase mediates mechanically induced COX-2 and PG EP4 receptor expression in podocytes: implications for the actin cytoskeleton
Am J Physiol Renal Physiol, April 1, 2004; 286(4): F693 - F701.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. R. James, D. Tang, A. Ingram, H. Ly, K. Thai, L. Cai, and J. W. Scholey
Flux Through the Hexosamine Pathway Is a Determinant of Nuclear Factor {kappa}B- Dependent Promoter Activation
Diabetes, April 1, 2002; 51(4): 1146 - 1156.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Krepinsky, A. J. Ingram, L. James, H. Ly, K. Thai, D. C. Cattran, J. A. Miller, and J. W. Scholey
17beta -Estradiol Modulates Mechanical Strain-induced MAPK Activation in Mesangial Cells
J. Biol. Chem., March 8, 2002; 277(11): 9387 - 9394.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online